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Biochemical characteristics of the granulosis viruses dna of common cabbage worm pieris rapae and pieris brassicae



Biochemical characteristics of the granulosis viruses dna of common cabbage worm pieris rapae and pieris brassicae



Korean Journal of Applied Entomology 30(2): 138-143



This study was carried out to acquire some basic biochemical informations on the granulosis virus (GV) DNA of Pieris rapae and Pieris brassicae. The thermal denaturation temperature (Tm) and G+C content of the DNA of the viruses were 83.7.degree.C and 35.5% for P. rapae GV, 84.0.degree.C and 35.9% for P. brassicae GV, respectively. There were some differences in the DNA fragmentation patterns of the two GV's produced by digestion with restriction endonucleases such as EcoR I,BamH I and HindIII. The homology between the two DNA's was calculated to be 97.0%. The size of the genome was estimated to be 103 kbp for P. rapae GV and 108 kbp for P. brassicae GV.

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Accession: 007058261

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