Biosynthesis of a membrane adhesion zone fraction throughout the cell cycle of Escherichia coli
Joseleau-Petit, D.; Kepes, F.; Peutat, L.; D'Ari, R.; Rothfield, L.I.
Journal of Bacteriology 172(11): 6573-6575
Synchronized cells of Escherichia coli were pulsed-labeled with [3H]leucine and subjected to membrane fractionation to determine whether a fraction that is enriched for membrane-murein adhesion zones (fraction OML) was preferentially generated at specific times during the cell cycle, as previously suggested from studies of lkyD and cha mutants. Contrary to this prediction, the experiments showed that OML was formed continuously during the division cycle.