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Calcium activates actin filament sliding on scallop myosin but inhibits that on physarum myosin



Calcium activates actin filament sliding on scallop myosin but inhibits that on physarum myosin



Journal of Biochemistry (Tokyo) 106(6): 955-957



The actin-activated ATPase activity of Physarum myosin has been shown to be inhibited by .mu.M levels of Ca2+, the mode of which is in contrast to the activating effect of Ca2+ on scallop myosin (Kohama, K. (1987) Adv. Biophys. 23, 149-182 for a review). To determine if Ca2+ regulates ATP-dependent sliding between actin and the myosins, fluorescent actin-filaments were allowed to move on the myosins fixed to a glass surface. The movement on Physarum and scallop myosins was inhibited and activated, respectively, by Ca2+. For this myoxin-linked regulation to occur for Physarum myosin, myosin phosphorylation was shown to be a prerequisite.

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Accession: 007074085

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