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Callus formation and plant regeneration from protoplasts derived from suspension culture of rice oryza sativa l. cultivar taipei 177


Callus formation and plant regeneration from protoplasts derived from suspension culture of rice oryza sativa l. cultivar taipei 177



Plant Breeding 105(2): 95-100



ISSN/ISBN: 0179-9541

DOI: 10.1111/j.1439-0523.1990.tb00460.x

Protoplasts were isolated from young inflorescence-derived suspension cultures of a japonica rice cultivar 'Taipei 177'. The isolated protoplasts which were cultured either in liquid, agar on Sea plaque agarose underwent sustained division. Maximum plating efficiency of 1.06% occurred in a medium containing macroelements of KM, microelements and vitamins of B5, 0.5% Sea plaque agarose, 1.0 mg/l of 2,4-D, and glucose as an osmotic stabilizer. Green and albinno plants were regenerated from the protocalli in MS semisolid medium containing 4 mg/l BAP, 0.5 mg/l NAA and 500 mg/l casein hydrolysate (MS18-2).

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Accession: 007076110

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