EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Cell loss in bisected mouse, sheep and cow embryos






Theriogenology 32(1): 115-122

Cell loss in bisected mouse, sheep and cow embryos

The purpose of this experiment was to determine the loss of cells during the splitting of embryos. The experiment was carried out on early, middle and late stage mouse blastocysts as well as on late stage sheep and cow blastocysts. The embryos were bisected with a metal mioroscalpel. Manipulation of the embryos was done in phosphate buffered saline (PBS) solution with 20% fetal calf serum (FCS) at +37 degrees C or in PBS solution free of Ca(++) and Mg(++) ions. To determine the number of cells present, intact embryos and demi-embryos were placed in acridine orange solution and examined under a fluorescent microscope. The average number of cells in early, middle and late stage mouse blastocysts was 39.4, 54.1 and 71.9, respectively. The loss of cells caused by splitting was 6.4, 7.6 and 9.9, respectively. The average number of cells in late stage sheep blastocysts was 115.3 and in late stage cow blastocysts it was 138.5. In sheep and cows, the loss of cells due to splitting the embryos in a PBS solution free of Ca(++) and Mg(++) ions did not differ from that observed in a PBS solution with 20% FCS. Similarly, bisection carried out at +37 degrees C did not result in lower cell losses, than that done at room temperature.

(PDF same-day service: $19.90)

Accession: 007086042

PMID: 16726658

DOI: 10.1016/0093-691x(89)90527-x



Related references

Development of reconstituted mouse embryos produced from the cytoplast of bisected oocytes or pronuclear-stage embryos and single blastomeres of 2-cell stage embryos. Theriogenology 38(5): 921-934, 1992

A mouse model for improving cell survival of bisected cattle embryos. Agricultural & Food Science in Finland 5(5): 529-534, 1996

Cell allocation in twin half mouse embryos bisected at the 8-cell stage: implications for preimplantation diagnosis. Molecular Reproduction and Development 36(1): 16-22, 1993

The developmental potential of mouse demi-embryos bisected from frozen storage embryos by vitrification. Journal of Chinese Society of Animal Science 17(3-4): 21-28, 1988

Development of reconstituted mouse embryos produced from bisected and electrofused pronuclear-stage embryos. Theriogenology 37(3): 655-663, 1992

Auto transplantation of bisected embryos in the sheep. Japanese Journal of Fertility & Sterility 34(2): 153-156, 1989

Factors affecting the survival of bisected sheep embryos in vivo. Theriogenology 36(3): 379-388, 1991

Freezing experiments with normal and bisected mouse embryos. Cryobiology 25(6): 0-568, 1988

Sex ratio in mouse chimeras derived from aggregation of five bisected embryos. Japanese Journal of Zootechnical Science 59(10): 868-873, 1988

The developmental potential of bisected frozen thawed mouse embryos. Japanese Journal of Animal Reproduction 32(3): 153-155, 1986