Cellulase secretion by immobilized protoplasts of trichoderma reesei

Marinova, D.N.; Atev, A.P.

Acta Biotechnologica 12(4): 319-323

1992


DOI: 10.1002/abio.370120413
Accession: 007087684

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Abstract
Protoplasts from Trichoderma reesei were immobilized in alginate and induced to produce cellulase (endoglucanase and .beta.-glucosidase) enzymes. The specific activities of the synthesized enzymes were higher in immobilized protoplasts than in both free and immobilized mycelial. Immobilized protoplasts show an enhnaced rate of exocellular .beta.-glucosidase production compared to intact mycelia due to the lack of cell wall. The ratio of the exocellular/intracellular .beta.-glucosidase was 5.9 for immobilized protoplasts and 0.32 for free mycelia.

Cellulase secretion by immobilized protoplasts of trichoderma reesei