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Changes in protein synthesis protein degradation and nucleic acid content in response to the beta adrenergic agonist clenbuterol in muscle of rats


Archiv fuer Tierzucht 35(4): 421-429
Changes in protein synthesis protein degradation and nucleic acid content in response to the beta adrenergic agonist clenbuterol in muscle of rats
The present study was designed to determine the effect of clenbuterol, a beta-adrenergic agonist, on muscle growth, protein synthesis, activity of calcium-dependent proteinase (CDP) as well as nucleic acid- and protein content in different muscles of rats. Clenbuterol administered orally as a feed additive (5 and 18 ppm respectively) for 12 days increased growth rate of the rats only slightly, but had a significant effect on muscle weights (19-25%). The protein synthesis studied using a cell-free translation assay was reduced in parts. Total protein content (7 - 25%) and RNA content (25 %) were increased. The DNA content remained unchanged. CDP-activity was reduced by 10 - 14% significantly upon clenbuterol feeding. The possible mode of action of clenbuterol was discussed to be caused by stimulated protein synthesis on the pretranslational level and inhibited calcium-dependent protein degradation. Both effects seem to be responsible for the observed muscle hypertrophy demonstrated by decreased nucleus-cytoplasm-ratios (24 - 28%).


Accession: 007093757



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