Section 8
Chapter 7,097

Characterisation of potential adhesins of the bacterium Pasteuria penetrans, and of putative receptors on the cuticle of Meloidogyne incognita, a nematode host

Persidis, A.; Lay, J.G.; Manousis, T.; Bishop, A.H.; Ellar, D.J.

Journal of Cell Science 100: 613-622


ISSN/ISBN: 0021-9533
PMID: 1808209
Accession: 007096883

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Pasteuria penetrans spores were fragmented by glass bead vortexing, producing exosporial membranes and spore fragments, which consisted of fibre bundles. Both exosporia and spore fragments are capable of host-specific attachment to the cuticle of Meloidogyne incognita, a root-knot nematode host. Putative M. incognita receptors appear to be soluble in .beta.-mercaptoethanol (BME) but not SDS, and are also sensitive to tryptic digestion and deglycosylation by endoglycosidase F. Polyclonal antibodies against intact spores and spore fragments inhibited attachment up to 25% and Fab' fragments of antispore antibodies produced 100% inhibition. The antibodies, however, did not show preferential staining of particular spore structures in thin section immunolabelling studies. Exposure of Pasteuria penetrans spores to HCl or urea-SDS-dithiothreitol renders them incapable of attachment to their host juveniles and extensively disrupts fibres that surround the spore core. Protein extracts from spore fragments or from exosporial membranes are identical and urea-BME extracts from either structure, but not SDS extracts, can inhibit the attachment of spores to juveniles by 60-80%. An inhibitory BME extract from spores fragments was analyzed by anion-exchange chromatography and adsorption onto host cuticle followed by immunoblotting. It appeared to contain six potential spore adhesins of approximate Mr 24-29, 38-47, 59, 89, 126, and 190 (.times. 103). Lectin affinity blotting with wheat germ agglutinin and concanavalin A showed that all of these proteins bear terminal N-acetylglucosamine residues and the 38-47 kDa band also bears terminal Glc/Man residues. A BME extract from strain Pp 1 inhibited the attachment of strain PNG to M. incognita and vice versa and the PNG strain had a greater amount of two proteins of 98 and 126 kDa than the Pp 1 extract.

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