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Characteristics and biologic significance of change in dna content of rat liver cells during hepatocarcinogenesis



Characteristics and biologic significance of change in dna content of rat liver cells during hepatocarcinogenesis



Zhonghua Zhongliu Zazhi 14(1): 20-23



DNA content of hepatocellular carcinoma (HCC) induced by diethylnitrosamine (DENA) in Wistar rats was quantitatively measured using flow cytometry. In normal adult rats, hepatocytes with tetraploid (4C) DNA content constitute the major cellular population (72.1%), whereas diploid (2C) hepatocytes were of a quate small number (16.9%). Under the persistent effect of DENA, the atavistic proliferation of 2C hepatocytes was obviously increased at the cirrhotic stage, which, followed the pre-cirrhotic stage, and replaced 4C hepatocytes to become the major cellular population. HCC at its early canceration stage was characterized by predominant in of DNA 2C cells, and HCC at its progression stage showed a selective and advantageous growth of aneupllid(AN) stemline cells. The whole hepatocarcinogenesis course showed a change of DNA stemline from 4C to 2C to AN, which might be the essential biologic kinetic mechanism of the development and progression of HCC. It is also suggested that HCC at early and progression stages are characterized by predominance of DNA 2c and AN hepatocytes.

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Accession: 007097016

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PMID: 1396040



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