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Characteristics and carbon metabolism of mesophyll and paraveinal mesophyll protoplasts from leaves of non nodulated glycine max



Characteristics and carbon metabolism of mesophyll and paraveinal mesophyll protoplasts from leaves of non nodulated glycine max



Plant Science (Shannon) 66(2): 167-172



The paraveinal mesophyll (PVM) is a spatially, morphologically and ultrastructurally specialized tissue in soybean leaves. It accounts for about 18% of the mesophyll volume as determined by stereological measurements and represents a significant cellular compartment, equal in size to that of the spongy mesophyll. Mesophyll cells (MC), their protoplasts (MP) and PVM protoplasts (PVMP) were isolated from leaves of non-nodulated plants and their physical characteristics and relative activity in some aspects of carbon metabolism were determined. PVMP were about twice the diameter and seven times the volume of MP: they had smaller and 2/3 fewer chloroplasts, and about one third the chlorophyll content of MP. Ribulose 1,5-bisphosphate carboxylase (RuBPCase) activity and 14CO2 fixation rates of PVMP were 40% and 30%, respectively, of the values measured in MP on a protoplast basis. PVMP showed net oxygen consumption (240 nmol O2 (106 protoplasts)-1 h-1) in the light with 10 mM bicarbonate, while MP showed the expected photosynthetic oxygen evolution (89 nmol O2 (106 protoplasts)-1 h-1) under similar conditions. The dark respiration rate of PVMP was about six times greater on a chlorophyll basis, three times greater on a protoplast basis and one point seven times greater on a cytoplasmic volume basis than the rate measured in MP. The data show that the PVM plays a minor role in carbon fixation in soybean leaves and supports the hypothesis that one of its primary roles is as a cellular pathway for metabolite transport within the leaf.

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Accession: 007097017

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