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Characterization of l leucine transport in the opportunistic yeasts malassezia furfur and malassezia pachydermatis



Characterization of l leucine transport in the opportunistic yeasts malassezia furfur and malassezia pachydermatis



Current Microbiology 23(6): 315-320



The lipophilic yeasts Malassezia furfur and M. pachydermatis show an initial rapid uptake of L-leucine followed by slower steady-state rates. At least two independent transport systems for L-leucine were present in both species. The high-affinity system for M. furfur had KT of 0.047 .mu.M with a Jmax of 222 fM/min/106 cells (65 pM/min/mg dry weight), whereas for M. pachydermatis the KT was 0.067 .mu.M with a Jmax of 709 fM/min/106 cells (89 pM/min/dry weight). The low-affinity system for M. furfur had a KT of 646 .mu.M with a Jmax of 1.62 pM/min/106 cells (0.5 nM/min/mg dry weight) and that of M. pachydermatis had a KT of 3.3 .mu.M with a Jmax of 9.97 pM/min/106 cells (1.3 nM/min/mg dry weight). Both transport systems were energy-dependent. Cells incubated with Tween 80 showed L-leucine uptake via both transport systems. Cells incubated with a combination of glucose (1%) and Tween 80 (0.01%) showed decreased transport rates for the high-affinity system for both species as compared with cells incubated only with glucose. The low-affinity transport system of both species in the presence of glucose plus Tween 80 showed an initial rapid uptake followed by greater efflux than influx of L-leucine. L-Leucine demonstrated binding to Tween 80, but the major effect of Tween 80 on membrane transport in Malassezia appears to be on the efflux of transported molecules.

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