Characterization of the antigen identified by Po66. A monoclonal antibody raised against a lung squamous cell carcinoma

Martin, A.; Pellen, P.; Guitton, C.; Youinou, P.; Collet, B.; Desrues, B.; Bourel, D.; Dazord, L.; Toujas, L.

Cancer Immunology Immunotherapy Cii 29(2): 118-124

1989


ISSN/ISBN: 0340-7004
PMID: 2541906
Accession: 007103739

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Abstract
The mouse monoclonal antibody (mAb) Po66 has been shown in previous work to be localized in nude mice xenografts of human lung tumours when injected intravenously [Dazord L et al. (1987) Cancer Immunol Immunother 24: 263-268] and to be suitable for the scintigraphic detection of lung cancers in patients [Dazord L, et al. (1987) in Klapdor (ed). New tumour markers and their monoclonal antibodies. Georg Thieme, Stuttgart, New York, pp 44-450]. The nature of the antigen recognized by Po66 has been investigated in the present work and comparisons are made with antigens recognized by other mAbs prepared in the laboratory. These mAbs were raised either against lung squamous cell carcinoma (mAbs Po43, Po60), or against a bronchio-alveolar carcinoma (mAbs BAM33, BAM45, BAM54 and BAM69). Radioiodinated purified Po66 did not compete for cell binding with any other mAb. All Po and BAM mAbs reacted with tumour cells both cultured in vitro and grown in vivo. They recognized cytoplasmic antigens as judged by immunofluorescence examinaiton of fixed cells or by immunoperoxidase stainign of cancer tissues, bu tcould never be visualized by immunofluorescence on the surface membrane of culture cells. The mAbs of teh BAM series reacted with vimentin as demonstrated by immunofluorescence stainign, showing alterations in the aspect of the filaments under the effect of colchicin. Radiolabelled mAbs Po43, BAM33 and BAM45 bound to partially purified cytoplasmic cytoskeleton components. In contrast, Po66 was never seen associated with intermediary filaments. The sensitivity to enzyme digestion of the antigen associated with Po66 was studied in comparison with those associated with Po43, BAM33 and BAM45. All antigens were sensitive to protease digestion while only the Po66-identified antigen was sensitive to periodate, neuraminidase and .alpha.-fucosidase. Thus, mAb Po66 identified an antigen of 47 kDa (as determined before) present in the cytoplasm but not related to the cytoskeleton, not detected on the cell surface and glycoproteic in nature.