+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterization of the gut juice and hemolymph proteins of mamestra brassicae l. pieris brassicae l. and spodoptera littoralis boisd. larvae and susceptibility to bacillus thuringiensis var thuringiensis crystal toxins



Characterization of the gut juice and hemolymph proteins of mamestra brassicae l. pieris brassicae l. and spodoptera littoralis boisd. larvae and susceptibility to bacillus thuringiensis var thuringiensis crystal toxins



Mededelingen van de Faculteit Landbouwwetenschappen Universiteit Gent 53(1): 255-260



The susceptibility of the tested larvae to the crystal toxin of Bacillus thuringiensis var. thuringiensis was as follows: Pieris brassicae .gtoreq. Mamestra brassicae > Spodoptera littoralis. The pH value of regurgitated gut juice was alkaline (8.32 to 9.91) in last instars and the protein concentration 7.04 for P. brassicae, 2.96 for M. brassicae and 3.44 mg/ml for S. littoralis. Polyacrylamide electrophoresis on a 7.5% gel under pH 8.9 buffered conditions revealed that some gut juice proteins migrated to the anode, others to the cathode. Polyacrylamide electrophoresis on a 5-10% gradient gel under pH 8.9 buffered conditions separated more than 23, 16 and 19 bands with a M.W. between and 70 and 800 kDa in the haemolymph of M. brassicae, P. brassicae and S. littoralis, respectively. Some glyco- and lipoproteins could be determined in the haemolymph: in M. brassicae: 1 glyco- and 2 lipoproteins; in P. brassicae: 4 glyco- and 4 lipoproteins; and in S. littoralis: 3 glyco- and 4 lipoproteins.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 007104240

Download citation: RISBibTeXText


Related references

Determination of prostatic secretion in rats effect of neurotransmitters and testosterone. Prostate 18(4): 289-302, 1991

Determination of propylthiouracil in human breast milk by direct injection high performance liquid chromatography. Journal of Liquid Chromatography 14(14): 2745-2754, 1991

Occurrence of a T cell lymphoma in a patient with chronic myeloid leukemia treated with alpha interferon. Leukemia and Lymphoma 19(5-6): 515-517, 1995

Host specificity of sarcoptes scabiei var canis acari sarcoptidae and the role of host odor. Journal of Medical Entomology 25(1): 52-56, 1988

Enzymatic hydrolysis of the crystals of Bacillus thuringiensis by the proteases of Pieris brassicae I Preparation and fractionation of the lysates. Journal of Invertebrate Pathology 9(3): 310-321, 1967

Susceptibility of major groups of tamil nadu india to diseases 1. psoriasis vulgaris. Journal of Genetics 68(2): 75-92, 1989

A recombinant hybrid peptide composed of AAF adhesin of enteroaggregative Escherichia coli and Shiga toxin B subunit elicits protective immune response in mice. European Journal of Clinical Microbiology and Infectious Diseases 28(11): 1311-1316, 2009

Fate of terbutryn in macrophyte-free and macrophyte-containing farm ponds. Weed Research 21(2): 59-70, 1981

Histopathological effects of Bacillus thuringiensis Berl. on larvae on Anagasta kuhniella Zeller. "Redia" giornale di zoologia3(63): 19-23, 1980

Teratological development in Tulipa gesneriana var. spathulata. Nuovo Giorn Bot Ital 62(3/4): 448-454, 1955

Simple amplification of GC-rich repetitive sequences in Bos taurus rDNA with PCR. Journal of Hunan Agricultural University 31(6): 602-604, 2005

Histopathological effects of Bacillus thuringiensis -endotoxin on the Malpighian tubules of Pieris canidia larva. Zoological Studies 33(3): 192-199, 1994

Effect of endotoxine of Escherichia coli on the level of 2,3,-diphosphoglycerate in rabbits. Veterinarno meditsinski nauki = Veterinary science1(7-8): 106-112, 1984

Toxic effect of copper studied by electro cardiogram in carp cyprinus carpio. Bulletin de la Societe d'Histoire Naturelle de Toulouse 112(1-2): 188-198, 1976

Mode of action of AgNO3 in maximizing vase life of Dendrobium 'Pompadour' flowers. Postharvest Biology and Technology 5(1-2): 109-117, 1995