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Chapter 7,108

Chemical modification of pseudomonas aeruginosa bacterial lectin

Pal, R.; Ray, S.; Chatterjee, B.P.

Biochemical Archives 5(4): 331-338

1989

ISSN/ISBN: 0749-5331
Accession: 007107043
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Two Pseudomonas aeruginosa (Habs serotype) letins, Pa-H 2a I and Pa-H 2a II, having specificity for D-galactose, and L-fucose and D-mannose, respectively isolated from Pa-H 2a were subjected to various chemical modification in order to ascertain the amino acid residues responsible for their carbohydrate-binding property. Modification of tryptophan, aspartic acid, glutamic acid and thiol groups led to a complete or significant loss of their activity indicating the involvement of these amino acid residues in the saccharide-binding property of the lectins. However, modification of lysine and tyrosine had no effect on their activity. The c.d. spectra showed the presence of an identical pattern of conformation in the native and modified Pa-H 2a I and Pa-H 2a II lectins confirming that the loss in activity was due to modification only.

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