Chromosomal study on the genetic variants regenerated from tissue cultures of allium wakegi

Kim, H.H.; Seo, B.B.; Lee, J.Y.

Korean Journal of Genetics 12(2): 104-112


Accession: 007112890

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Giemsa C-banded karyotypes on the 200 regenerated A. wakegi were analyzed. About 92.5% of the regenerated plants were amphihaploid plants, and the C-banding patterns of these plants were almost same as that of the common species. But four types of variants could be classified on the basis of morphological characteristics and C-banding patterns. Amphidiploid variants which is composed of 5% of the total regenerated plants showed doubled C-banding karyotypes of amphihaploid one. Four types of the amphidiploid variants exhibited a chromosomal rearrangement identified by C-band distributions. Hypoamphidiploid variants of 2n = 28 were also obtained, but they showed low fitness to the environmental factors. Amphidiploid plants showed better viability, growth and larger bulb than the common cultivar plants.