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Cloning and expression of cellulomonas fimi beta glucosidase genes in escherichia coli


Cloning and expression of cellulomonas fimi beta glucosidase genes in escherichia coli



Enzyme and Microbial Technology 11(5): 313-316



ISSN/ISBN: 0141-0229

Two .beta.-glucosidase genes of Cellulomonas fimi were cloned in Escherichia coli using pUC9 as a vector. The genes were expressed in E. coli from lacZ promoter of pUC9. One of the two genes encoded a .beta.-glucosidase which hydrolysed only p-nitrophenyl-.beta.-D-glucopyranoside, while the .beta.-glucosidase encoded by the other gene hydrolysed both p-nitrophenyl-.beta.-D-glucopyranoside and cellobiose. The latter enzyme produced only glucose form cellobiose.

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Accession: 007125097

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