+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Combination of alkaline phosphatase anti alkaline phosphatase apaap and avidin biotin alkaline phosphatase complex abap techniques for amplification of immunocytochemical staining of human testicular tissue



Combination of alkaline phosphatase anti alkaline phosphatase apaap and avidin biotin alkaline phosphatase complex abap techniques for amplification of immunocytochemical staining of human testicular tissue



Andrologia 23(5): 353-356



An amplification procedure was developed for the visualization of antigens in human testis using monoclonal antibodies against desmin and vimentin. The technique combines the high sensitive and specific APAAP- and ABAP-methods. Depending on the quality of the antibodies used and the processing of the material prior to the immunocytochemical staining the amplification technique may be applied either as a single APAAP and ABAP- or as a double APAAP and ABAP-combination. Especially after the double amplification reaction a distinct increase of the staining intensity of the vimentin- (in Sertoli cells, myofibroblasts of the lamina propria, and fibroblasts of the interstitium) and desmin- (in myofibroblasts of the lamina propria and smooth muscle cells of the blood vessels) like immunoreactivity was observed. If different diazonium salts were used for the visualization of the alkaline phosphatase activity (e.g. Fast Red TR Salt, Fast Blue BB Salt) desmin- and vimentin-like immunoreactivity can be demonstrated in the same tissue section in a double sequential staining approach. For double staining, the alkaline phosphatase technique may be combined successfully with a technique or a combination that uses peroxide as a marker.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 007131270

Download citation: RISBibTeXText

PMID: 1724878


Related references

A one-day double-labelling technique for tissue specimens: Immunogold-silver staining forin situhybridization combined with alkaline phosphatase-anti-alkaline phosphatase (APAAP) immunohistochemistry for antigens. Journal of Molecular Histology 28(2): 133-140, 1996

A one-day double-labelling technique for tissue specimens: immunogold-silver staining for in situ hybridization combined with alkaline phosphatase-anti-alkaline phosphatase (APAAP) immunohistochemistry for antigens. Histochemical Journal 28(2): 133-134, 1996

Immunocytochemical labelling of hematological samples using the alkaline phosphatase anti alkaline phosphatase apaap technique. Australian & New Zealand Journal of Medicine 17(4 Suppl. 2): 542, 1987

Identification of proliferating lymphocyte subpopulations by combined alkaline phosphatase anti-alkaline phosphatase (APAAP) staining and autoradiography. Journal of Immunological Methods 90(2): 283-285, 1986

Comparative analysis of biotin intranuclear inclusions of gestational endometrium utilizing the alkaline phosphatase anti-alkaline phosphatase, avidin biotin complex and the peroxidase anti-peroxidase immunodetection systems. Pathology International 46(Suppl. 1): 675, 1996

Monoclonal alkaline phosphatase-anti-alkaline phosphatase (APAAP) complex: production of antibody, optimization of activity, and use in immunostaining. Journal of Histochemistry and Cytochemistry 36(2): 137-143, 1988

A dual immunohistochemical phenotype analysis of tumor cell adherent lymphocytes by a two color modification of the monoclonal alkaline phosphatase anti alkaline phosphatase complex method apaap. Tissue Antigens 33(2): 365, 1989

Color contrast stain of two different lymphocyte subpopulations a two color modification of alkaline phosphatase monoclonal anti alkaline phosphatase apaap complex technique. Blut 55(4): 317, 1987

Comparative studies on alkaline phosphatase , alkaline phosphatase amplification horseradish peroxidase , penicillinase and avidin-biotin penicillinase amplification ELISA in detection of maize streak virus in maize plants and Cicadulina mbila insect vector. Scientific Research And Essays: 11, 524-530, 2008

Immunoenzymatic labelling pattern of dihydrofolate reductase using alkaline phosphatase anti alkaline phosphatase apaap method in hemic cells. Acta Histochemica et Cytochemica 21(5): 499-506, 1988

Monoclonal antibody study of Philadelphia chromosome-positive blastic leukemias using the alkaline phosphatase anti-alkaline phosphatase (APAAP) technic. American Journal of Clinical Pathology 85(5): 564-572, 1986

Quantitative analysis of megakaryocytic proliferation in cases with myelodysplastic syndromes mds utilizing apaap alkaline phosphatase anti alkaline phosphatase method. Journal of Tokyo Medical College 47(3): 463-471, 1989

Detection of chlamydial inclusions in cell culture or biopsy tissue by alkaline phosphatase-anti-alkaline phosphatase staining. Journal of Clinical Microbiology 25(10): 1864-1867, 1987

Clinical significance of serum high-molecular-mass alkaline phosphatase, alkaline phosphatase-lipoprotein-X complex, and intestinal variant alkaline phosphatase. Journal Of Clinical Laboratory Analysis. 8(3): 172-176, 1994

Sensitivity and specificity of indirect immunofluorescence and Grocott-technique in comparison with immunocytology (alkaline phosphatase anti alkaline phosphatase = APAAP) for the diagnosis of Pneumocystis carinii in broncho-alveolar lavage (BAL). European Journal of Medical Research 3(12): 559-563, 1999