EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Comparison of alpha helix stability in peptides having a negatively or positively charged residue block attached either to the amino or carboxyl terminus of an alpha helix the electrostatic contribution and anisotropic stability of the alpha helix


Comparison of alpha helix stability in peptides having a negatively or positively charged residue block attached either to the amino or carboxyl terminus of an alpha helix the electrostatic contribution and anisotropic stability of the alpha helix



Biopolymers 28(5): 995-1010



ISSN/ISBN: 0006-3525

An estimation of the thermodynamic effects of a charged random coil, which is attached either to the N- or C-terminus of polyalanine, upon .alpha.-helix stability is attempted. A temperature-induced helix-coil transition of Ala20Lys20Phe and Lys20Ala20Phe was studied under various conditions of salt concentration and pH. By combining the results with previous ones for Ala20Glu20Phe and Glu20Ala20Phe, which have opposite electric charges to the present system [S. Ihara et al. (1982) Biopolymers 21, 131-145], the free energy of the coil to helix transition of the polyalanine block could be separated into two terms.sbd.one term for the electrostatic interaction of electric charges in the random-coil block with the .alpha.-helix dipole, and a second term for the intrinsic stability of the helix. The first term indicates the significance of the helix dipole-charge interactions, which affects the helix stability depending on the attaching side of the charged block and on the sign of the charges. This clearly shows the anisotropic stability of the .alpha.-helix. Furthermore, analysis of the dependence of these thermodynamic quantities on salt concentrations showed, assuming that the effect of the attached electric charges was symmetric (in other words, the absolute values of the electrostatic interaction terms were independent of the sign of electric charges), that the intrinsic stability of the .alpha.-helix was dependent on which side of the helix was attached to the random coil: a random coil attached to the N-terminus of the .alpha.-helix had little effect while that attached to a C-terminal significantly destabilized the helix.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 007138628

Download citation: RISBibTeXText

Related references

Comparison of alpha-helix stability in peptides having a negatively or positively charged residue block attached either to the N- or C-terminus of an alpha-helix: the electrostatic contribution and anisotropic stability of the alpha-helix. Biopolymers 28(5): 995-1009, 1989

Factors governing 3(10)-helix vs alpha-helix formation in peptides: percentage of C(alpha)-tetrasubstituted alpha-amino acid residues and sequence dependence. Biopolymers 64(5): 236-245, 2002

Charged histidine affects alpha-helix stability at all positions in the helix by interacting with the backbone charges. Proceedings of the National Academy of Sciences of the United States of America 90(23): 11337-11340, 1993

Further studies of the helix dipole model: effects of a free alpha-NH3+ or alpha-COO- group on helix stability. Proteins 5(1): 1-7, 1989

Contribution of the 30/36 hydrophobic contact at the C-terminus of the alpha-helix to the stability of the ubiquitin molecule. Biochemistry 39(33): 10275-10283, 2000

Effect of the N1 residue on the stability of the alpha-helix for all 20 amino acids. Protein Science: a Publication of the Protein Society 10(3): 463-470, 2001

Effect of the N2 residue on the stability of the alpha-helix for all 20 amino acids. Protein Science: a Publication of the Protein Society 10(7): 1305-1311, 2001

Influence of preformed alpha-helix and alpha-helix induction on the activity of cationic antimicrobial peptides. Journal of Peptide Research: Official Journal of the American Peptide Society 52(2): 81-88, 1998

The electrostatic potential of the alpha helix (electrostatic potential/alpha-helix/secondary structure/helix dipole). Biophysical Chemistry 11(2): 133-136, 1980

Calculation of electrostatic effects at the amino terminus of an alpha helix. Biophysical Journal 67(6): 2251-2260, 1994

Conformational studies on host-guest peptides containing chiral alpha-methyl-alpha-amino acids. Comparison of the helix-inducing potential of alpha-aminoisobutyric acid, (S)-2-ethylalanine and (S)-2-methylserine. International Journal of Peptide and Protein Research 32(5): 344-351, 1988

Design and synthesis of novel nonpolar host peptides for the determination of the 3-10- and alpha-helix compatibilities of alpha-amino acid building blocks: An assessment of alpha,alpha-disubstituted glycines. Biopolymers 42(5): 575-626, 1997

The role of glycine (residue 89) in the central helix of EF-hand protein troponin-C exposed following amino-terminal alpha-helix deletion. Protein Science: a Publication of the Protein Society 3(11): 2089-2096, 1994

Helix formation in preorganized beta/gamma-peptide foldamers: hydrogen-bond analogy to the alpha-helix without alpha-amino acid residues. Journal of the American Chemical Society 132(23): 7868-7869, 2010

Functional roles of amino acid residues involved in forming the alpha-helix-turn-alpha-helix operator DNA binding motif of Tet repressor from Tn10. Proteins 14(2): 168-177, 1992