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Comparison of ELISA with electron microscopy, and PAGE for the detection of human rotavirus in stools



Comparison of ELISA with electron microscopy, and PAGE for the detection of human rotavirus in stools



Journal of the Medical Association of Thailand 71(8): 436-442



A total of 363 diarrheaic stool specimens were collected from May 1985 to April 1986 from children aged 5 days to 12 years and 4 months who had acute diarrhea and came to Bangkok [Thailand] children's hospital for treatment. Rotaviruses were found in 27.27 per cent of the patients by enzyme-linked immunosorbent assay (ELISA). The highest incidence of the rotavirus diarrhea was in the month of January which was cool and dry and was found among the children aged between 6 months and 2 years old. The incidence between males and females was 1.54:1. Most of the rotavirus diarrheaic patients were from low income families. Nine different RNA electropherotypes were found among the rotaviruses isolated. Seventy per cent were of long pattern of which electropherotype A predominated throughout the year course of study. ELISA is the most sensitive method for the clinical diagnostic purpose while the electropherotyping is suitable for epidemiological study.

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Accession: 007139865

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PMID: 2855233


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