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Comparison of long and short forms of the prolactin receptor on prolactin induced milk protein gene transcription



Comparison of long and short forms of the prolactin receptor on prolactin induced milk protein gene transcription



Proceedings of the National Academy of Sciences of the United States of America 88(3): 824-828



The biological activities of long and short forms of the prolactin receptor have been compared. These two receptors expressed in mammalian cells were shown to bind prolactin with equal high affinity. The ability of these different forms to transduce the hormonal message was estimated by their capacity to stimulate transcription by using the promoter of a milk protein gene fused to the chloramphenicol acetyltransferase (CAT) coding sequences. Experiments were performed in serum-free conditions to avoid the effect of lactogenic factors present in serum. An .apprxeq. 17-fold induction of CAT activity was obtained in the presence of prolactin when the long form of the prolactin receptor was expressed, whereas no induction was observed when the short form was expressed. The present results clearly establish that only the long form of the prolactin receptor is involved in milk protein gene transcription.

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Accession: 007140995

Download citation: RISBibTeXText

PMID: 1992473

DOI: 10.2307/2356078


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