+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Comparison of the chromatographic properties of sterols select additional steroids and triterpenoids gravity flow column liquid chromatography thin layer chromatography gas liquid chromatography and high performance liquid chromatography



Comparison of the chromatographic properties of sterols select additional steroids and triterpenoids gravity flow column liquid chromatography thin layer chromatography gas liquid chromatography and high performance liquid chromatography



Journal of Chromatography 452: 377-398



The chromatographic properties of approximately 100 sterols, select steroids of plant origin (sapogenins and steroidal alkaloids) has been evaluated in this laboratory by monitoring their elution characteristics in adsorption (gravity column and thin-layer methods with and without the addition of silver nitrate), gas and reversed-phase high-performance liquid chromatography. The utility of each methodology to act in one or another chromatographic mode-separation, radiochemical purifiction, quantitation and structural elucidation, is discussed. The importance of the tilt of the -OH group at C-3 as well as the polarity, size, and shape of the rest of the molecule as it effects the hydrogen-bonding ability of the -OH group is demonstrated through changes in chromatographic behavior that result from the step-wise production of double bonds, methyl, bromo, oxygen, nitrogen and cyclopropyl groups into 5.alpha.-cholestanol. An independent aid in the structure identification and quantitation of the compounds was use of a multiple-wavelength diode array detector in which different wavelengths of the UV spectrum (200-400 nm) were simultaneously monitored following passage of the sample through a reversed phase C18 column.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 007142930

Download citation: RISBibTeXText

PMID: 3243853


Related references

Chromatographic analysis of fatty acid dimers comparison of gas liquid chromatography high performance liquid chromatography and thin layer chromatography with flame ionization detection. Journal of Chromatography 354: 283-292, 1986

Chromatographic studies on fatty acid dimers gas liquid chromatography high performance liquid chromatography and thin layer chromatography. Journal of the American Oil Chemists' Society 66(9): 1353-1359, 1989

Phytochemical studies on male and female flowers of Hagenia abyssinica by column chromatography, thin-layer chromatography and high-performance liquid chromatography. Analytical Proceedings 27(7): 178-179, 1990

Pesticides High performance liquid chromatography, gas chromatography, gas chromatography-mass chromatography, thin layer chromatography. Journal of chromatography library: 2435-458, 1983

Comparative study of roots of Angelica sinensis and related umbelliferous drugs by thin layer chromatography, high-performance liquid chromatography, and liquid chromatography-mass spectrometry. Phytochemical Analysis 9(6): 283-290, 1998

Separation of some metabolically important aromatic n acyl amino acids of the benzoyl and cinnamoyl series by thin layer chromatography gas liquid chromatography and high performance liquid chromatography. Journal of Chromatography 234(1): 141-156, 1982

Determination of furanochromones and pyranocoumarins in drugs and Ammi visnaga fruits by combined solid-phase extraction-high-performance liquid chromatography and thin-layer chromatography-high-performance liquid chromatography. Journal of Chromatography. a 797(1-2): 305-309, 1998

Lipophilicity measurement of benzodiazepine-receptor ligands by reversed-phase liquid chromatography. Comparison between high-performance liquid and thin-layer chromatography. Journal of Chromatography 447(2): 404-409, 1988

Comparison of chromatographic high performance liquid chromatography and thin layer chromatography with conventional methods for the measurement of chlorophylls in oceanic waters. Ergebnisse der Limnologie (16): 123-124, 1982

Separation and identification of some common isomeric plant triterpenoids by thin-layer chromatography and high-performance liquid chromatography. Journal of Chromatography. a 1216(38): 6662-6670, 2009

Analysis of flavonol aglycones and terpenelactones in Ginkgo biloba extract: A comparison of high-performance thin-layer chromatography and column high-performance liquid chromatography. Journal of Aoac International 90(5): 1203-1209, 2007

Separation of mevalonate phosphates and isopentenyl pyrophosphate by thin-layer chromatography and of short-chain prenyl phosphates by ion-pair chromatography on a high-performance liquid chromatography column. Methods in Enzymology 111: 248-252, 1985

Determination of zearalenone in cornflakes and other corn based foods by thin layer chromatography, high pressure liquid chromatography, and gas liquid chromatography. 1978

Secondary metabolites isolation in natural products chemistry: comparison of two semipreparative chromatographic techniques (high pressure liquid chromatography and high performance thin-layer chromatography). Journal of Chromatography. a 1325: 256-260, 2014

Reversed phase high performance thin layer chromatography and column liquid chromatography of chlorophylls and their derivatives. Journal of Chromatography 408: 181-190, 1987