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Conjugal rp4 transfer between pseudomonads in soil and recovery of rp4 plasmid dna from soil



Conjugal rp4 transfer between pseudomonads in soil and recovery of rp4 plasmid dna from soil



Systematic & Applied Microbiology 11(2): 223-227



Plasmid RP4 transfer via conjugation between Pseudomonas donor and recipient cells was studied in both sterile and non-sterile soil samples under a variety of conditions. Transconjugants were recovered from sterile soil incubated at 18.degree. C (adjusted to 60% were holding capacity) as early as 6 h after introduction of donor and recipient cells. However, no transconjugants were detected over a 48 h period in soil samples incubated at 10.degree. C under identical conditions. In addition, RP4 transfer was not detected in soil samples incubated at 25.degree. C and maintained at 20% of the soil's water holding capacity. Pseudomonas sp. donor cells containing RP4 introduced in soil were easily recovered (98.1%) using a differential centrifugation and plating technique. These procedures and information may be useful in studying genetic interactions in the soil environment.

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Accession: 007152862

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DOI: 10.1016/S0723-2020(89)80065-7


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