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Constructing balancer chromosomes for genetic screens in Drosophila hydei

Constructing balancer chromosomes for genetic screens in Drosophila hydei

Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 83(6-7): 821-826

We used a screen for maternally generated late embryonic lethals as a new method for the isolation of inversions that are suitable for the balancing of mutations in Drosophila hydei. The recovery of several inversions by this method demonstrates that female meiosis in D. hydei apparently differs from meiosis in female D. melanogaster, since in D. hydei the defective chromosomes which are generated by a single crossing-over within a paracentric inversion can be recovered via the egg nucleus. In addition, the classic method of crossing-over suppression were used in order to isolate more inversions and to improve the balancing capacities of inversions. We succeeded in constructing chromosomes that allow the balancing of mutations on nearly the whole genome of D. hydei. We discuss here whether or not this method is suited for application to other organisms.

Accession: 007154421

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PMID: 24202759

DOI: 10.1007/BF00226703

Download PDF Full Text: Constructing balancer chromosomes for genetic screens in Drosophila hydei

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