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Corticosteroid analysis by hplc uv facilitated by use of an injector mounted extraction column

Corticosteroid analysis by hplc uv facilitated by use of an injector mounted extraction column

Journal of Liquid Chromatography 14(7): 1257-1266

A method for integrating solid phase extraction of corticosteroids from biological samples with normal operation of reversed-phase HPLC is described. This method uses an extraction column mounted in place of the sample loop of a conventional injection valve to separate corticosteroids from some interfering compounds, and to effectively concentrate steroids from dilute samples. Both manual and fully automated chromatographs using this principle are described. A specific application to measurement of corticosterone in a rabbit serum preparation allows routine measurement of as little as 300 pg corticosterone per sample.

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