+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Corticosteroid analysis by hplc uv facilitated by use of an injector mounted extraction column



Corticosteroid analysis by hplc uv facilitated by use of an injector mounted extraction column



Journal of Liquid Chromatography 14(7): 1257-1266



A method for integrating solid phase extraction of corticosteroids from biological samples with normal operation of reversed-phase HPLC is described. This method uses an extraction column mounted in place of the sample loop of a conventional injection valve to separate corticosteroids from some interfering compounds, and to effectively concentrate steroids from dilute samples. Both manual and fully automated chromatographs using this principle are described. A specific application to measurement of corticosterone in a rabbit serum preparation allows routine measurement of as little as 300 pg corticosterone per sample.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 007165092

Download citation: RISBibTeXText


Related references

Pressurized solvent extraction and monolithic column-HPLC/DAD analysis of anthocyanins in red cabbage. Talanta 74(5): 1218-1223, 2008

Monolithic spin column: a new extraction device for analysis of drugs in urine and serum by GC/MS and HPLC/MS. Journal of Aoac International 94(3): 765-774, 2011

Simultaneous column chromatographic extraction and purification of abscisic acid in peanut plants for direct HPLC analysis. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences 1002: 277-284, 2016

Examination of the dry column procedure of lipid extraction for analysis of tocopherols in human milk by normal phase hplc. Federation Proceedings 46(3): 896, 1987

Determination of antihyperglycemic biguanides in serum and urine using an ion-pair solid-phase extraction technique followed by HPLC-UV on a pentafluorophenylpropyl column and on an octadecyl column. Biomedical Chromatography 20(11): 1200-1205, 2006

Hplc analysis of physostigmine and eseroline in human serum using solid phase extraction and post column fluorescence ion pair techniques. Journal of Pharmaceutical Sciences 76(11): S15, 1987

Enantioselective analysis of R- and S-propafenone in plasma by HPLC applying column switching and liquid-liquid extraction. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences 877(29): 3489-3494, 2009

Quantitative determination of cationic surfactants in sewage sludges and sediments by supercritical fluid extraction and HPLC applying post-column ion-pair extraction. Abstracts of Papers American Chemical Society 205(1-2): ENVR 64, 1993

Analysis of cocaine, benzoylecgonine, ecgonine methyl ester, ethylcocaine and norcocaine in human urine using HPLC with post-column ion-pair extraction and fluorescence detection. Journal of Pharmaceutical and Biomedical Analysis 10(10-12): 943-948, 1992

HPLC analysis of pindolol enantiomers in human serum using a reversed phase chiral column and solid phase extraction. Pharmaceutical Research (New York) 11(10 SUPPL ): S21, 1994

The use of a monolithic column to improve the simultaneous determination of four cephalosporin antibiotics in pharmaceuticals and body fluids by HPLC after solid phase extraction--a comparison with a conventional reversed-phase silica-based column. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences 809(1): 175-182, 2004

Totally automated sequential analysis of tricyclic antidepressants by solid phase extraction module system and reversed phase HPLC with a base deactivated C-18 column. Clinical Chemistry 42(6 PART 2): S222, 1996

Effective on-line extraction of drugs from plasma using a restricted-access media column in column-switching HPLC equipped with a dilution system: application to the simultaneous determination of ER-118585 and its metabolites in canine plasma. Journal of Pharmaceutical and Biomedical Analysis 49(5): 1250-1255, 2009

Method development of combining pressurized liquid extraction and off-line HPLC fractionation with a porous graphite carbon column for the analysis of polychlorinated dibenzo-p-dioxins and dibenzofurans PCDD/Fs in environmental samples. International Journal of Environmental Analytical Chemistry 93(12): 1296-1310, 2013

HPLC analysis of salinomycin in human plasma using pre-column oxidation and automated heart cut column switching. Journal Of Pharmaceutical & Biomedical Analysis. 11(9): 823-827, 1993