+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Corticosteroid receptors in cells derived from rat brain microvessels: mRNA identification and aldosterone binding



Corticosteroid receptors in cells derived from rat brain microvessels: mRNA identification and aldosterone binding



American Journal of Physiology 262(1 Pt 1): C156-C163



B7 is a cell clone derived from rat brain microvessels. Expression of an amiloride-sensitive cationic channel has been recently established in these cells. In this study, the polymerase chain reaction (PCR) was used to amplify definite segments of mineralocorticoid and glucocorticoid receptor mRNA in B7 cells. Aldosterone binding was also characterized. Two classes of sites were detected. Aldosterone exhibited a high affinity for type I sites [dissociation constant (Kd) approximately 0.3 nM] and a lower one for type II sites (Kd approximately 20 nM). RU 28362, a highly specific glucocorticoid agonist, did not compete for type I sites. RU 28362 and dexamethasone were better competitors for type II sites than aldosterone. The sedimentation coefficients of aldosterone type I and type II complexes were approximately 9S. These characteristics are close to the one exhibited by aldosterone type I and type II receptors in rat kidney and other target tissues. In intact B7 cells, aldosterone binding expressed as number of acceptor sites per cell was higher (approximately 41,000 for type II and 8,800 for type I) than in the soluble cellular extract (approximately 18,000 for type II and 1,000 for type I).

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 007165122

Download citation: RISBibTeXText

PMID: 1733230


Related references

Binding of aldosterone to cytoplasmic and nuclear corticosteroid brain receptors in rats with different types of behaviour. Biology Bulletin of the Russian Academy of Sciences 27(5): 536-539, 2000

Binding of aldosterone with rat brain corticosteroid receptors: effect of typological features of behavior and stress. Biulleten' Eksperimental'noi Biologii i Meditsiny 120(7): 49-51, 1995

Effects of stress and types of animal behavior on binding of aldosterone to brain corticosteroid receptors. Biology Bulletin of the Russian Academy of Sciences 26(5): 511-514, 1999

Aldosterone binding with corticosteroid brain receptors in rats: Effect of behavioral typology and stress. Bulletin of Experimental Biology and Medicine 120(1): 694-696, 1995

Binding of aldosterone with cytoplasmic and brain nuclear corticosteroid receptors in rats with various types of behavior. Izvestiia Akademii Nauk. Seriia Biologicheskaia .(5): 636-640, 2000

The binding of [3H]aldosterone by brain corticosteroid receptors in rats with different individual-typological behavioral characteristics. Izvestiia Akademii Nauk. Seriia Biologicheskaia .(3): 344-350, 1996

Stimulation of sex hormone-binding globulin mRNA and attenuation of corticosteroid-binding globulin mRNA by triiodothyronine in human hepatoma cells. European Journal of Endocrinology 130(2): 166-170, 1994

Transformation and nuclear translocation of brain type i corticosteroid receptors complexed with the mineralocorticoid antagonist zk 91587 aldosterone or dexamethasone. Journal of Steroid Biochemistry & Molecular Biology 42(5): 515-520, 1992

Functional identification of adenylate cyclase-coupled adenosine receptors in rat brain microvessels. European Journal of Pharmacology 85(2): 177-184, 1982

The expression of native and oxidized LDL receptors in brain microvessels is specifically enhanced by astrocytes-derived soluble factor(s). Febs Letters 522(1-3): 19-23, 2002

Identification of GTP binding proteins in brain microvessels and their role in phosphoinositide turnover. Biochemical and Biophysical Research Communications 195(2): 952-957, 1993

Identification of P-glycoprotein co-fractionating proteins and specific binding partners in rat brain microvessels. Journal of Neurochemistry 134(2): 200-210, 2015

Comparisons of P-glycoprotein expression in isolated rat brain microvessels and in primary cultures of endothelial cells derived from microvasculature of rat brain, epididymal fat pad and from aorta. Febs Letters. 374(2): 179-183, 1995

Functional brain-specific microvessels from iPSC-derived human brain microvascular endothelial cells: the role of matrix composition on monolayer formation. Fluids and Barriers of the Cns 15(1): 7, 2018

Identification of beta-adrenergic receptor binding sites in rat brain microvessels, using [125I]iodohydroxybenzylpindolol. Journal of Neurochemistry 36(4): 1383-1388, 1981