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Corticotropin like intermediary lobe peptide as a marker of alternate pro opiomelanocortin processing in acth producing non pituitary tumors

Corticotropin like intermediary lobe peptide as a marker of alternate pro opiomelanocortin processing in acth producing non pituitary tumors

Clinical Endocrinology 31(6): 691-700

In order to evaluate which of human (h) corticotrophin-like intermediary lobe peptide (CLIP) or h.beta.MSH5-22 was the better marker of alternate pro-opiomelanocortin (POMC) processing, both peptides were simultaneously sought in the same tissue extracts from a normal human pituitary, six corticotrophic adenomas, and four non-pituitary tumours responsible for an ectopic ACTH syndrome. Human CLIP was detected using a combination of gel exclusion chromatography and two different radioimmunoassays (RIAs): a mid-ACTH RIA which recognized ACTH but not CLIP, and a COOH-ACTH RIA which recognized both molecules. Human .beta.MSH5-22 had been measured previously. Neither hCLIP nor h.beta.MSH5-22 were detected in the normal or tumoural pituitaries. The four non-pituitary tumours, in contrast, contained both peptides; the hCLIP and h.beta.MSH5-22 ratios (CLIP/ CLIP +ACTH and h.beta.MSH5-22/h.beta.MSH5-22-h.gamma.LPH) ranged from 40 to 94% and from 24 to 46%, respectively. In a given tissue the hCLIP ratio was always higher than the h.beta.MSH5-22 ratio. hCLIP is therefore the better marker of alternate POMC processing.

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Accession: 007165217

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PMID: 2560687

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