+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Cortisol alters gene expression during involution of the rat ventral prostate

Cortisol alters gene expression during involution of the rat ventral prostate

Molecular Endocrinology 3(4): 703-708

The ability of high doses of cortisol to retard the involution process in the rat ventral prostate was related to alterations in the pattern of gene expression. Poly(A)+ RNA preparations from the prostates of noncastrated, castrated, and castrated rats injected daily for 7 days with cortisol were compared by Northern blot hybridizations for the relative expression of genes associated with cell differentiation and maintenance (the C1 prostatic steroid binding protein gene and alpha-tubulin), with cell death (TRPM-2, hsp 70, and c-fos), and with hormone regulation (the androgen and glucocorticoid receptors). As anticipated, the concentration of C1 mRNA in the prostate fell to less than 4% of that in the noncastrated controls within 4 days after castration and was nearly undetectable after 7 days. This decline was retarded by cortisol treatment of 7-day castrated animals which sustained the level of C1 transcripts at approximately 50% of control. While the pattern of expression of alpha-tubulin indicated some minor fluctuations, with the highest level occurring 7 days after castration, the prostates of the cortisol-treated group had essentially the same concentration of this mRNA as the noncastrates. Cortisol also modified the expression of genes associated with prostatic cell death. The large increase in prostatic TRPM-2 mRNA, seen 7 days after castration, was reduced by over 80% after treatment with the glucocorticoid. Although not as abundantly expressed as TRPM-2, the castration-induced levels of transcripts for both hsp 70 and the protooncogene c-fos were substantially reduced by cortisol.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 007165320

Download citation: RISBibTeXText

PMID: 2498651

DOI: 10.1210/mend-3-4-703

Related references

Finasteride treatment alters MMP-2 and -9 gene expression and activity in the rat ventral prostate. International Journal of Andrology 33(1): E114-E122, 2010

Thanatogen expression during involution of the rat ventral prostate after castration. Journal of Andrology 15(3): 200-211, 1994

IGFBP-5 expression is associated with involution of the ventral prostate in castrated and finasteride-treated rats. Journal of Andrology 0(SUPPL ): P52, 1996

Selective enhancement of expression of class-Mu glutathione S-transferase genes during involution of rat ventral prostate induced by androgen withdrawal. Yonago Acta Medica 39(3): 151-154, 1996

In utero exposure to tributyltin alters the expression of E-cadherin and localization of claudin-1 in intercellular junctions of the rat ventral prostate. Molecular Reproduction and Development 74(4): 455-467, 2006

WNT1 gene expression alters in heterogeneous population of prostate cancer cells; decreased expression pattern observed in CD133+/CD44+ prostate cancer stem cell spheroids. Journal of B.U.On. 19(1): 207-214, 2014

Increased activity of plasminogen activators during involution of the rat ventral prostate. Biochemical Journal 221(1): 171-178, 1984

Decrement of blood flow precedes the involution of the ventral prostate in the rat after castration. Urological Research. 25(5): 309-314, 1997

The relationship between androgen and differential expression of c erb b2 neu neu epidermal growth factor receptor egfr cytokeratin 8 ck 8 and the prostate binding protein gene c 3 by rat ventral prostate rvp during postnatal development. Journal of Urology 147(4 SUPPL): 318A, 1992

Hormonal control of gene expression in rat ventral prostate effects of androgen withdrawal on the expression of growth related genes. Journal of Urology 137(4 PART 2): 127A, 1987

Age-associated changes in histology and gene-expression profile in the rat ventral prostate. Laboratory Investigation 83(5): 743-757, 2003

Expression of a 74-kDa nuclear factor 1 (NF1) protein is induced in mouse mammary gland involution. Involution-enhanced occupation of a twin NF1 binding element in the testosterone-repressed prostate message-2/clusterin promoter. Journal of Biological Chemistry 271(47): 29688-29697, 1996

Transient hypertrophy of the ventral prostate and coagulating glands and accelerated thymic involution following pinealectomy in the mouse. Texas Reports on Biology and Medicine 29(4): 579-586, 1971

Increased levels of clusterin (SGP-2) mRNA and protein accompany rat ventral prostate involution following finasteride treatment. Journal of Endocrinology 167(2): 197-204, 2000

The ultrastructure of the accessory sex organs of the male rat. II. The post-castration involution of the ventral, lateral and the dorsal prostate. Zeitschrift für Zellforschung und Mikroskopische Anatomie 144(2): 167-178, 1973