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Cortisol and cortisone production in rat and mouse adrenal incubations



Cortisol and cortisone production in rat and mouse adrenal incubations



Journal of Steroid Biochemistry & Molecular Biology 37(2): 279-284



The presence of 17.alpha.-hydroxylase in rodent adrenals is debated. The presence in blood of mice of 11-deoxycortisol together with the absence of cortisol is well known. We demonstrated here the in vitro synthesis of 17.alpha.-hydroxyprogesterone and cortisol from [3H]progesterone in rat and mouse adrenals. We have shown that these syntheses represented 45 and 28% of those of 11-deoxycorticosterone and corticosterone, repectively, from progesterone. These data clearly suggest the presence of a 17.alpha.-hydroxylase activity in vitro in these rodents adrenals. In addition, a noticeable synthesis of cortisol (0.87-1.57% per mg tissue, i.e., 52-64% per incubation flask) from 11-deoxycortisol was also observed and was inhibited by 0.1-0.3 .mu.mol of Metyrapone and SKF 12185. These results allow to underline that the adrenals of rat and mouse, two species commonly used in laboratory experiments, may be used for in vitro investigations on cortisol metabolism from exogenous radioactive precursors.

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Accession: 007165327

Download citation: RISBibTeXText

PMID: 2268561

DOI: 10.1016/0960-0760(90)90339-M


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