+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Cortisol and cortisone production in rat and mouse adrenal incubations

Cortisol and cortisone production in rat and mouse adrenal incubations

Journal of Steroid Biochemistry & Molecular Biology 37(2): 279-284

The presence of 17.alpha.-hydroxylase in rodent adrenals is debated. The presence in blood of mice of 11-deoxycortisol together with the absence of cortisol is well known. We demonstrated here the in vitro synthesis of 17.alpha.-hydroxyprogesterone and cortisol from [3H]progesterone in rat and mouse adrenals. We have shown that these syntheses represented 45 and 28% of those of 11-deoxycorticosterone and corticosterone, repectively, from progesterone. These data clearly suggest the presence of a 17.alpha.-hydroxylase activity in vitro in these rodents adrenals. In addition, a noticeable synthesis of cortisol (0.87-1.57% per mg tissue, i.e., 52-64% per incubation flask) from 11-deoxycortisol was also observed and was inhibited by 0.1-0.3 .mu.mol of Metyrapone and SKF 12185. These results allow to underline that the adrenals of rat and mouse, two species commonly used in laboratory experiments, may be used for in vitro investigations on cortisol metabolism from exogenous radioactive precursors.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 007165327

Download citation: RISBibTeXText

PMID: 2268561

DOI: 10.1016/0960-0760(90)90339-M

Related references

Cortisol cortico sterone and deoxy cortisol assay in plasma with the trans cortisone cortisol carbon 14 method with glass paper chromatography use in cases of adrenal cortical tumor. Zeitschrift fuer Klinische Chemie und Klinische Biochemie 8(4): 436-437, 1970

Chemical transformations of steroids by adrenal perfusion. V. Blood incubations and perfusions with cortisone. Journal of Biological Chemistry 203(1): 469-487, 1953

Clinical significance of cortisone and cortisone/cortisol ratio in evaluating children with adrenal diseases. Clinica Chimica Acta. 256(1): 1-11, 1996

A possible defect in the inter-conversion between cortisone and cortisol in prepubertal patients with congenital adrenal hyperplasia receiving cortisone acetate therapy. Journal of Steroid Biochemistry and Molecular Biology 39(4a): 461-470, 1991

The balance of cortisol-cortisone interconversion is shifted towards cortisol in neonates with congenital adrenal hyperplasia due to 21-hydroxylase deficiency. Journal of Steroid Biochemistry and Molecular Biology 143: 386-391, 2014

The postprandial rise in plasma cortisol in men is mediated by macronutrient-specific stimulation of adrenal and extra-adrenal cortisol production. Journal of Clinical Endocrinology and Metabolism 99(1): 160-168, 2014

Plasma concentration of depot cortisone following cortisone therapy and the influence of this substance on endogenous cortisol production. Wiener Klinische Wochenschrift 82(21): 394-396, 1970

Ontogeny of the adrenal gland in the spiny mouse, with particular reference to production of the steroids cortisol and dehydroepiandrosterone. Endocrinology 154(3): 1190-1201, 2013

Differences between concentrations of salivary cortisol and cortisone and of free cortisol and of cortisone in plasma during pregnancy and postpartum. Clinical Chemistry 36(1): 70-75, 1990

Splanchnic cortisol production occurs in humans: evidence for conversion of cortisone to cortisol via the 11-beta hydroxysteroid dehydrogenase (11beta-hsd) type 1 pathway. Diabetes 53(8): 2051-2059, 2004

Intraindividual stability of cortisol and cortisone and the ratio of cortisol to cortisone in saliva, urine and hair. Steroids 118: 61-67, 2016

Analysis of cortisol, cortisone and dexamethasone in human serum using liquid chromatography tandem mass spectrometry and assessment of cortisol: cortisone ratios in patients with impaired kidney function. Clinica Chimica Acta; International Journal of Clinical Chemistry 412(13-14): 1221-1228, 2011

Plasma and adrenal cortisol and cortisone concentrations during puberty in guinea pig. Comptes Rendus Des Seances De La Societe De Biologie Et De Ses Filiales0-12: 1324-1330, 1974

Daily salivary cortisol and cortisone rhythm in patients with adrenal incidentaloma. Endocrine 59(3): 510-519, 2017

Plasma cortisol and cortisone concentrations following postoperative adrenal steroid replacement. Surgery 73(3): 429-433, 1973