+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Cosmid derived map of escherichia coli strain bhb2600 in comparison to the map of strain w3110



Cosmid derived map of escherichia coli strain bhb2600 in comparison to the map of strain w3110



Nucleic Acids Research 17(13): 5057-5070



A physical map for the genome of E. coli K12 strain BHB2600 was constructed by use of 570 cloned DNA elements (CDEs) withdrawn from a cosmid library. Dot blot hybridisation was applied to establish contig interrelations with subsequent fine mapping achieved by analysis of EcoR1 restriction patterns on Southern blots. The derived map covers nearly 95% of the E. coli genome resulting in 12 minor gaps. It may be compared to the almost complete map for strain W3110 of Kohara et al. (1). Except for one tiny gap (lpp, 36.5') remaining gaps in BHB2600 do not coincide with those in W3110 so that both maps complement each other establishing an essentially complete clone represented map. Besides numerous minute differences (site and fragment gains and losses) both strains harbour at differing positions extended rearrangements flanked by mutually inverted repetitive elements, in our case insertion elements (IS1 and IS5).

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 007165482

Download citation: RISBibTeXText


Related references

Cosmid-derived map of E.coli strain Bhb2600 in comparison to the map of strain W3110. Nucleic Acids Research 17(13): 5057-5070, 1989

Cosmid-derived map of E. coli strain BHB2600 in comparison to the map of strain W3110. Nucleic Acids Research 17(13): 5057-5069, 1989

Death of the Escherichia coli K-12 strain W3110 in soil and water. Applied and Environmental Microbiology 62(11): 4114-4120, 1996

The synthesis of dna by membrane dna complexes from escherichia coli strain b r and escherichia coli strain bs 1 after exposure to uv light a comparison with the effects of ionizing radiation. International Journal of Radiation Biology & Related Studies in Physics Chemistry & Medicine 26(3): 277-284, 1974

Comparison of mutagenic efficiency of decay of phosphorus 32 incorporated in escherichia coli strain wp 2 and escherichia coli strain wp 2s cells. Acta Microbiologica Polonica Series A Microbiologia Generalis 7(2): 99-105, 1975

The tdcE gene in Escherichia coli strain W3110 is separated from the rest of the tdc operon by insertion of IS5 elements. Dna Sequence 9(3): 183-188, 1999

Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome. Journal of Bacteriology 171(2): 1214-1218, 1989

Transcriptome analysis of a shikimic acid producing strain of Escherichia coli W3110 grown under carbon- and phosphate-limited conditions. Journal of Biotechnology 126(4): 528-545, 2006

Dna synthesis during the cell division cycle of escherichia coli a comparison of escherichia coli strain b r escherichia coli k 12 and escherichia coli 15t under conditions of slow growth. Abstracts of the Annual Meeting of the American Society for Microbiology 73: 69, 1973

Metabolic engineering of Escherichia coli W3110 strain by incorporating genome-level modifications and synthetic plasmid modules to enhance L-Dopa production from glycerol. Preparative Biochemistry and Biotechnology 2018: 1-12, 2018

Structural and biochemical examination of ghosts derived from a deep rough (heptose-deficient lipopolysaccharide) strain and a smooth strain of Escherichia coli. Canadian Journal of Microbiology 25(4): 436-446, 1979

Comparison of the mutagenic effects of sulfur 35 decay in escherichia coli strain wp 2 and strain wp 2s. Acta Microbiologica Polonica Series A Microbiologia Generalis 7(1): 37-40, 1975

Penicillin-binding proteins of Escherichia coli. Comparison of a strain carrying an R-factor and the parent strain. Biochimica et Biophysica Acta 491(1): 223-231, 1977

Membrane-bound penicillin-binding proteins of Escherichia coli. Comparison of a strain carrying an R-factor and the parent strain. Journal of Antibiotics 31(12): 1283-1291, 1978

Increased hydrogen production by Escherichia coli strain HD701 in comparison with the wild-type parent strain MC4100. Enzyme & Microbial Technology 33(2-3): 185-189, 13 August, 2003