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Countercurrent plunge cooling a new approach to increase reproducibility in the quick freezing of biological tissue

Countercurrent plunge cooling a new approach to increase reproducibility in the quick freezing of biological tissue

Journal of Microscopy (Oxford) 156(2): 173-182

Conditions fulfilling all the accepted criteria for optimum cryoquenching are realized for the first time in a plunge freezing device. Samples are plunged down through a smoothly rising cryogen stream that is maintained within .+-. 0.25 K of a preselected temperature. Dry nitrogen gas at ambient temperature removes evaporated cryogen molecules from the plunging path immediately above the liquid coolant to prevent pre-cooling of the sample. Symmetrical instrument design and conditions of flow ensure symmetrical freezing under optimum conditions in a totally repeatable fashion. The device has been used with halocarbons, propane and ethane. It provides consistent cooling rates comparable with the bet so far obtained by any other plunging method.

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Accession: 007165929

Download citation: RISBibTeXText

DOI: 10.1111/j.1365-2818.1989.tb02916.x

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