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Counterflow affinity isotachophoresis on cellulose acetate membranes



Counterflow affinity isotachophoresis on cellulose acetate membranes



Electrophoresis 12(6): 414-419



Counterflow isotachophoresis on cellulose acetate membranes of human alpha-fetoprotein (AFP) was performed with concanavalin A, lentil lectin, and castor bean lectin driven by electroendosmotic counterflow. This counterflow caused a uniform stream of lectin to migrate towards the cathode against AFP with carrier ampholytes in steady-state position. Retardation of microheterogeneity forms bound to lectins was observed, giving results comparable to standard crossed affinity immunoelectrophoresis. Smaller amounts of lectins and more diluted samples of AFP could be used in the described method.

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Accession: 007165934

Download citation: RISBibTeXText

PMID: 1716198

DOI: 10.1002/elps.1150120606


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