Section 8
Chapter 7,167

Covalent immobilization of biomolecules onto polystyrene MicroWells for use in biospecific assays

Rasmussen, S.E.

Annales de Biologie Clinique 48(9): 647-650


ISSN/ISBN: 0003-3898
PMID: 2099117
Accession: 007166335

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Modification of polystyrene for higher binding capacity and/or for specific covalent immobilization of biomolecules is discussed. The benefit of covalent coupling of biomolecules onto a new commercially available surface type for covalent immobilization, CovaLink NH, is illustrated. The CovaLink NH solid phase has spacer arms covalently grafted onto the polystyrene solid phase, approximately 10(14) groups/cm2. Coupling procedures for covalent immobilization of biotin and peptides are demonstrated, and the advantage of using carbodiimide for coupling of carboxylic acid containing compounds is shown.

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