+ Site Statistics
References:
52,572,879
Abstracts:
28,705,754
PMIDs:
27,750,366
DOIs:
25,464,004
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Cytoplasmic membrane systems involved in bacterium release into soybean nodule cells as studied with two Bradyrhizobium japonicum mutant strains



Cytoplasmic membrane systems involved in bacterium release into soybean nodule cells as studied with two Bradyrhizobium japonicum mutant strains



European Journal of Cell Biology 49(1): 24-32



Two Bradyrhizobium japonicum, Tn5-induced, mutant strains, ML126 and ML150, were studied. Both induce host cell division to form normal-sized nodules that do not fix nitrogen and whose cells have very few bacteroids (Bar-). Early-infection (15 days post infection) cells have much endoplasmic reticulum (ER), numerous Golgi bodies, and large vacuoles that are probably secondary lysosomes. Later the cytoplasm of the host cells of both are dominated by hundreds of vesicles containing only finely fibrous material and that appear to originate by the degradation of the cell walls of the infection threads; they have been named "infection-thread wall degradation vesicles" (IWDV). Phosphotungstic acid-chromic acid (PACA) staining of thin sections shows that IWDV membranes and the plasma membranes of both the cells and infection threads usually stain quite intensely, while the membranes of other cell organelles do not. The membranes of the few symbiosomes present in the mutants also stain with PACA. This evidence suggests that largely the host-cell plasma membrane gives rise to both the vesicle and symbiosome membranes in these mutants. In cells induced by both mutants, ER appears to be deficient, a finding suggesting that an ER-synthesis signal is involved in the normal release process, that ER synthesis is prerequisite to a normal volume of release, and that insufficient ER can impair symbiosome formation. In the mutant-induced infections, normal lysosomes develop and engulf both symbiosomes and cytoplasmic vesicles, but the retardation of this activity is the probable cause of the cytoplasm becoming overloaded with vesicles.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 007176217

Download citation: RISBibTeXText

PMID: 2759102


Related references

Bradyrhizobium japonicum genes involved in soybean root-nodule development. Recognition in microbe plant symbiotic and pathogenic interactions edited by Ben Lugtenberg: 6, 1986

Bradyrhizobium japonicum genes involved in soybean root nodule development. Lugtenberg, B. Nato Asi (advanced Science Institutes) Series, Series H: Cell Biology, Vol. 4. Recognition In Microbe-Plant Symbiotic And Pathogenic Interactions; Nato Advanced Research Workshop, Biddinghuizen, Netherlands, May 11-16, . Xiii 449p. Springer-Verlag New York, Inc: Secaucus, New Jersey, Usa; Berlin, West Germany. Illus. 79-86, 1986, 1987

Sampling to the determine nodule occupancy of soybean Merr by Bradyrhizobium japonicum strains. Canadian Journal of Microbiology 44(8): 795-799, 1998

Bacterial genes involved in the communication between soybean and its root nodule symbiont, Bradyrhizobium japonicum. NATO ASI series: Series H: Cell biology6(36): 295-301, 1989

Visualizing the symbiosome: FESEM characterization of fegAB-, a Bradyrhizobium japonicum mutant defective in soybean nodule persistence. 2007

Nitrogen fixation in mutant soybean lines inoculated with two Bradyrhizobium japonicum strains. Agrochimica 49(5/6): 233-245, 2005

A dual-targeted soybean protein is involved in Bradyrhizobium japonicum infection of soybean root hair and cortical cells. Molecular Plant-Microbe Interactions 24(9): 1051-1060, 2012

Novel avidin-like protein from a root nodule symbiotic bacterium, Bradyrhizobium japonicum. Journal of Biological Chemistry 280(14): 13250-5, 2005

Predominant populations of indigenous soybean-nodulating Bradyrhizobium japonicum strains obtained from organic farming systems in Minnesota. Journal of Applied Microbiology 118(5): 1152-1164, 2016

Delayed nodule development in a succinate transport tn5 mutant of bradyrhizobium japonicum. Bothe, H , F J De Bruijn And W E Newton (Ed ) Nitrogen Fixation: Hundred Years After; 7th International Congress, Cologne, West Germany, March 13-20, Xxi+878p Vch Publishers, Inc : New York, New York, Usa; Vch Verlagsgesellschaft Mbh: Weinheim, West Germany; Gustav Fischer Verlag: Stuttgart, West Germany Illus 559, 1988

Delayed nodule development in a succinate transport mutant of bradyrhizobium japonicum. Journal of Plant Physiology 134(3): 276-283, 1989

Identification of Bradyrhizobium japonicum Nodule Isolates from Wisconsin Soybean Farms. Applied and Environmental Microbiology 51(3): 487-492, 1986

Identification of bradyrhizobium japonicum nodule isolates from wisconsin usa soybean farms. Applied & Environmental Microbiology 51(3): 487-492, 1986

Utilization of nitrate by bacteroids of Bradyrhizobium japonicum in the soybean root nodule. Planta 174(1): 51-58, 1988

Identification and characterization of a gene involved in nodule competition of Bradyrhizobium japonicum. Abstracts of the General Meeting of the American Society for Microbiology 93(0): 298, 1993