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Cytotoxic t lymphocyte ctl mediated cytolysis proceeds in the absence of sodium proton antiport activity regulation of cytosolic ph by the sodium proton antiport in a cloned ctl



Cytotoxic t lymphocyte ctl mediated cytolysis proceeds in the absence of sodium proton antiport activity regulation of cytosolic ph by the sodium proton antiport in a cloned ctl



Cellular Immunology 135(1): 208-221



Cytotoxic T lymphocyte (CTL)-mediated cytolosis of specifically bound target cells (TC) is thought to be triggered by cross-linking the T-cell antigen receptor (TcR). Biochemical events associated with TcR cross-linking include increased intracellular calcium levels [Ca2+]i, hydrolysis of phosphatidylinositol (PI), and in increase in intracellular pH [pH]i. Whereas CTL-mediated cytolysis of some TC is calcium-dependent, and PI hydrolysis is speculated to trigger the CTL lethal hit via activation of PKC, little is known about changes in [pH]i relating to activation of the lethal hit stage. We report regulation of [pH]i in a cloned CTL by the electroneutral Na+/H+ antiport during activation with PMA and specific antigen-bearing TC. Furthermore, using 5-(N-methyl-n-isobutyl) amiloride (MIBA), a potent antiport inhibitor, we demonstrate that Na+/H+ exchange is not required for activation of CTL cytolytic activity.

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Accession: 007176909

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