+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Demonstration of cytomegalovirus nucleic acids in the coronary arteries of transplanted hearts

Demonstration of cytomegalovirus nucleic acids in the coronary arteries of transplanted hearts

American Journal of Pathology 140(3): 739-747

Cytomegalovirus (CMV) infection has been associated with the development of accelerated arteriosclerosis in heart transplant recipients. This association prompted the authors to examine, using by in situ hybridization techniques, the coronary arteries of 19 patients who had received heart transplants for the presence of CMV nucleic acids. Two probes were employed on formalin-fixed paraffin-embedded tissues. The first was an S35-labeled nick translated probe derived from CMV DNA. CMV nucleic acids were identified in the arterial intima of 5 (45%) of 11 transplanted hearts examined with this probe. Hybridization to cells morphologically consistent with endothelial cells, lymphocytes, and smooth muscle cells was identified. Hybridization was not detected in any of the hearts when a control probe, a vector without CMV DNA, was employed. The second probe that was used was a tritium-labeled strand-specific riboprobe derived from the immediate early (IE1) gene of CMV. Using this second probe, hybridization was detected in the coronary arteries of three of five hearts that were positive with the DNA probe, in one of the hearts that was negative with the DNA probe, and in three (38%) of eight additional transplanted hearts. The pattern of hybridization was the same as that seen with the DNA probe, and no hybridization was detected when a control sense probe for the IE1 gene of CMV was employed. These results suggest that CMV nucleic acids are present in the coronary arteries of heart transplant recipients.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 007183463

Download citation: RISBibTeXText

PMID: 1312309

Related references

Angiographic appearance of coronary arteries from transplanted hearts predicts coronary flow reserve. Circulation 88(4 Part 2): I420, 1993

Cytomegalovirus nucleic acids in allografted hearts. Human Pathology 21(9): 981-982, 1990

Average peak flow velocity in the coronary arteries of transplanted hearts decreases with time. European Heart Journal 18(ABSTR Suppl. ): 14, 1997

Local gene expression in human coronary arteries from transplanted hearts analyzed by in situ hybridization. Circulation 82(4 Suppl. 3): III699, 1990

Inducible nitric oxide synthase expression in coronary arteries of transplanted human hearts with accelerated graft arteriosclerosis. American Journal of Pathology 151(4): 919-925, 1997

Inducible nitric oxide synthase is expressed in coronary arteries of transplanted human hearts with accelerated graft arteriosclerosis. Laboratory Investigation 76(1): 30A, 1997

Prominence of apolipoproteins B, (a), and E in the intimae of coronary arteries in transplanted human hearts: geographic relationship to vessel wall proteoglycans. Journal of Heart and Lung Transplantation 15(12): 1223-1232, 1996

Expression of allograft inflammatory factor-1 in vascular smooth muscle cells of coronary arteries and endomyocardial biopsies of transplanted human hearts. Journal of Heart & Lung Transplantation 19(1): 69-70, 2000

The number of nuclei and the thickness of the media and intima of the coronary arteries of normal hearts in the second and third decades of life and in hearts with coronary sclerosis and hypertension. Zeitschrift für Kardiologie 64(11): 995, 1975

Circulating nucleic acids of Chlamydia pneumoniae and cytomegalovirus in patients undergoing coronary angiography. Journal of Clinical Microbiology 39(2): 596-600, 2001

Coronary artery immunogenicity: a comparison between explanted recipient or donor hearts and transplanted hearts. Transplant Immunology 1(4): 294-301, 1993

Cocaine produces vasoconstriction of human coronary arteries in vitro studies using coronary arteries obtained from freshly explanted human hearts. Journal of the American College of Cardiology 15(2 Suppl. A): 215A, 1990

Hypophysectomy and nucleic acids. 2. Variations of the nucleic acid content in transplanted tumors in hypophysectomized animals. L' Ospedale Maggiore 49: 291-293, 1961

Demonstration of Chlamydophila pneumoniae, Mycoplasma pneumoniae, Cytomegalovirus, and Epstein-Barr virus in atherosclerotic coronary arteries, nonrheumatic calcific aortic and rheumatic stenotic mitral valves by polymerase chain reaction. Anadolu Kardiyoloji Dergisi 11(3): 237-243, 2011

Demonstration of autonomic nerves in transplanted arteries in rabbits. Journal of Microsurgery 3(1): 20-27, 1981