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Detection of antibodies to bovine leukemia virus in bovine milk samples with an ELISA involving two monoclonal antibodies



Detection of antibodies to bovine leukemia virus in bovine milk samples with an ELISA involving two monoclonal antibodies



Veterinary Microbiology 18(1): 89-93



An indirect enzyme-linked immunosorbent assay was used to detect antibody to bovine leukemia virus (BLV). In the assay the gp51 antigen was selectively adsorbed to the solid phase using a monoclonal capture antibody. Specific antibodies in milk or serum samples were revealed by the addition of a monoclonal antibovine IgG 1 conjugated to peroxidase. All milk samples from animals found positive by serum testing were shown to be positive. BLV-positive milk samples were detected when added to pools of negative samples.

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Accession: 007188912

Download citation: RISBibTeXText

PMID: 2847403

DOI: 10.1016/0378-1135(88)90118-6


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