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Determination of membrane lipid phase transition temperature from carbon nmr intensities



Determination of membrane lipid phase transition temperature from carbon nmr intensities



Journal of Biochemical & Biophysical Methods 20(4): 353-356



A new and simple approach for the determination of the temperature of gel-to-liquid crystalline phase transitions (TC) of biological (chloroplast) membrane lipids from 13C-NMR resonance intensities is proposed. The variation of intensity of a temperature-sensitive NMR resonance is monitored by recording the spectra of the sample at a range of temperatures. From such a series of spectra recorded at different temperatures, a temperature-insenstive resonance is located. Then the ratio of the intensity of the temperature-sensitive to the intensity of the temperature-insensitive resonance is calculated from each spectrum to even out the procedural error, if any. The values of this ratio at different temperatures, when plotted against sample temperature, shows a break at TC as confirmed by spin label ESR studies.

Accession: 007194219

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