+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Determination of membrane lipid phase transition temperature from carbon nmr intensities

Determination of membrane lipid phase transition temperature from carbon nmr intensities

Journal of Biochemical & Biophysical Methods 20(4): 353-356

A new and simple approach for the determination of the temperature of gel-to-liquid crystalline phase transitions (TC) of biological (chloroplast) membrane lipids from 13C-NMR resonance intensities is proposed. The variation of intensity of a temperature-sensitive NMR resonance is monitored by recording the spectra of the sample at a range of temperatures. From such a series of spectra recorded at different temperatures, a temperature-insenstive resonance is located. Then the ratio of the intensity of the temperature-sensitive to the intensity of the temperature-insensitive resonance is calculated from each spectrum to even out the procedural error, if any. The values of this ratio at different temperatures, when plotted against sample temperature, shows a break at TC as confirmed by spin label ESR studies.

Accession: 007194219

Download citation: RISBibTeXText

Related references

Determination of membrane lipid phase transition temperature from 13C-NMR intensities. Journal of Biochemical and Biophysical Methods 20(4): 353-356, 1990

Determination of lipid composition and thermal phase transition temperature in an enriched plasma membrane fraction from ram spermatozoa. Journal of Reproduction and Fertility 73(1): 285-294, 1985

Effect of quinidine on membrane properties. Depression of the lipid phase transition temperature and changes in the permeability of the lipid bilayer. Biochemical Pharmacology 32(9): 1467-1471, 1983

Membrane reconstitution below lipid phase transition temperature. Antolini, R , A Gliozzi And A Gorio (Ed ) Transport in Biomembranes: Model Systems And Reconstitution Xv+272p Raven Press: New York, N Y , Usa Illus P87-98, 1982

Superoxide anion radical induces lipid phase transitions that mimic temperature-induced phase-transition phenomenon of membrane lipids. Biochemical and Biophysical Research Communications 172(2): 601-606, 1990

Effect of temperature induced membrane lipid phase transition in recipients on conjugation in escherichia coli k 12. Abstracts of the Annual Meeting of the American Society for Microbiology 80: ABSTRACT 974, 1980

Line Tension Assists Membrane Permeation at the Transition Temperature in Mixed-Phase Lipid Bilayers. Journal of Physical Chemistry. B 120(45): 11740-11750, 2016

Exponential distribution of interimpulse current intervals in lipid bilayer membrane at phase transition temperature. Biofizika 44(5): 887-891, 2000

A low membrane lipid phase transition temperature is associated with a high cryotolerance of Lactobacillus delbrueckii subspecies bulgaricus CFL1. Journal of Dairy Science 96(9): 5591-5602, 2014

A new method for membrane reconstitution: fusion of protein-containing vesicles with planar bilayer membranes below lipid phase transition temperature. Biophysics of Structure and Mechanism 7(3): 131-137, 1981

Does membrane lipid profile explain chilling sensitivity and membrane lipid phase transition of spermatozoa and oocytes?. Cryo Letters 21(3): 179-186, 2002

Millisecond time scale temperature jumps in cryotransmission electron microscopy specimens a system for studying fast lipid phase transition and membrane fusion. FASEB Journal 6(1): A497, 1992

Temperature acclimation and phospho lipid phase transition in hypothalamic membrane phospho lipids of garden lizard calotes versicolor. Biochimica et Biophysica Acta 770(1): 7-14, 1984

Some aspects of lipid phase transition on membrane permeability and lipid protein association. Semenza, G And E Carafoli (Ed ) Federation Of European Biochemical Societies Symposium, No 42 Biochemistry Of Membrane Transport Zurich, Switzerland, July 18-23, 1976 Xix+669p Illus Springer-Verlag: Berlin, West Germany; New York, N Y , Usa ISBN 3-540-08082-1; ISBN 0-387-08082-1: 38-46, 1977

Does lipid profile explain chilling sensitivity and membrane lipid phase transition of spermatozoa and oocytes?. CryoLetters 21(3): 179-186, 2000