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Determination of the molecular weight of proteins by electrophoresis in slab gels with transverse pore gradient of crosslinked polyacrylamide in the absence of denaturing agents



Determination of the molecular weight of proteins by electrophoresis in slab gels with transverse pore gradient of crosslinked polyacrylamide in the absence of denaturing agents



Analytical Biochemistry 175(2): 544-547



The molecular weight of proteins under nondenaturing conditions can be determined through polyacrylamide electrophoresis by comparing their relative mobilities at different gel concentrations with the relative mobilities of standard proteins under the smae conditions (J. L. Hedrick and A. J. Smith (1968) Arch. Biochem. Biophys. 126, 155). This work describes a procedure that eliminates the need for several gels of different acrylamide concentrations with the use of a slab gel with a transverse pore gradient of crosslinked polyacrylamide.

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Accession: 007195785

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PMID: 3239780


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