+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

Different sequence elements are required for function of the cauliflower mosaic virus polyadenylation site in Saccharomyces cerevisiae compared with in plants



Different sequence elements are required for function of the cauliflower mosaic virus polyadenylation site in Saccharomyces cerevisiae compared with in plants



Molecular and Cellular Biology 12(5): 2322-2330



We show that the polyadenylation site derived from the plant cauliflower mosaic virus (CaMV) is specifically functional in the yeast Saccharomyces cerevisiae. The mRNA 3' endpoints were mapped at the same position in yeast cells as in plants, and the CaMV polyadenylation site was recognized in an orientation-dependent manner. Mutational analysis of the CaMV 3'-end-formation signal revealed that multiple elements are essential for proper activity in yeast cells, including two upstream elements that are situated more than 100 and 43 to 51 nucleotides upstream of the poly(A) addition site and the sequences at or near the poly(A) addition site. A comparison of the sequence elements that are essential for proper function of the CaMV signal in yeast cells and plants showed that both organisms require a distal and a proximal upstream element but that these sequence elements are not identical in yeast cells and plants. The key element for functioning of the CaMV signal in yeast cells is the sequence TAGTATGTA, which is similar to a sequence previously proposed to act in yeast cells as a bipartite signal, namely, TAG ... TATGTA. Deletion of this sequence in the CaMV polyadenylation signal abolished 3'-end formation in yeast cells, and a single point mutation in this motif reduced the activity of the CaMV signal to below 15%. These results indicate that the bipartite sequence element acts as a signal for 3'-end formation in yeast cells but only together with other cis-acting elements.

(PDF emailed within 0-6 h: $19.90)

Accession: 007208127

Download citation: RISBibTeXText

PMID: 1373813

DOI: 10.1128/mcb.12.5.2322


Related references

The polyadenylation signal of the cauliflower mosaic virus is recognized in the yeast saccharomyces cerevisiae. Journal of Cellular Biochemistry Supplement (15 PART G): 261, 1991

Effect of deletions in the cauliflower mosaic virus polyadenylation sequence on the choice of the polyadenylation sites in tobacco protoplasts. Molecular and General Genetics 226(1-2): 141-144, 1991

The cauliflower mosaic virus open reading frame VII product can be expressed in Saccharomyces cerevisiae but is not detected in infected plants. Journal of Virology 64(6): 2594-2598, 1990

Expression of cauliflower mosaic virus gene I in Saccharomyces cerevisiae. Research in Virology 142(4): 297-302, 1991

The cauliflower mosaic virus 35S promoter is regulated by cAMP in Saccharomyces cerevisiae. M G G: Molecular and general genetics 235(2-3): 365-372, 1992

Cyclic amp dependent regulation of the cauliflower mosaic virus sulfur 35 promoter in saccharomyces cerevisiae. Yeast 6(SPEC ISSUE): S301, 1990

Purification of the Saccharomyces cerevisiae cleavage/polyadenylation factor I. Separation into two components that are required for both cleavage and polyadenylation of mRNA 3' ends. Journal of Biological Chemistry 271(43): 27167-27175, 1996

DNA sequence elements required for transcription initiation of the Schizosaccharomyces pombe ADH gene in Saccharomyces cerevisiae. Molecular and General Genetics 223(3): 407-416, 1990

DNA sequence elements required for partitioning competence of the Saccharomyces cerevisiae 2-micron plasmid STB locus. Nucleic Acids Research 2018, 2018

In vitro cleavage of the simian virus 40 early polyadenylation site adjacent to a required downstream TG sequence. Molecular and Cellular Biology 6(12): 4734-4741, 1986

Sequence of a cauliflower mosaic virus strain infecting solanaceous plants. Gene (Amsterdam) 155(2): 305-306, 1995

Identification of an upstream activation sequence and other cis-acting elements required for transcription of COX6 from Saccharomyces cerevisiae. Molecular and Cellular Biology 9(12): 5350-5358, 1989

Elements upstream of the AAUAAA within the human immunodeficiency virus polyadenylation signal are required for efficient polyadenylation in vitro. Molecular and Cellular Biology 12(9): 3699-3705, 1992

A dissection of the cauliflower mosaic virus polyadenylation signal. Genes and Development 5(1): 141-149, 1991

Multiple cis regulatory elements for maximal expression of the cauliflower mosaic virus 35S promoter in transgenic plants. Plant Cell 1(1): 141-150, 1989