+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Differential distribution in villin and villin messenger rna in mouse intestinal epithelial cells

Differential distribution in villin and villin messenger rna in mouse intestinal epithelial cells

Differentiation 39(1): 51-58

The distribution of the mRNA encoding for villin, the major actin-binding protein of intestinal brush border, was studied during the differentiation of mouse intestinal epithelial cells and compared to the distribution of the protein. In situ hybridization using a cRNA clone specific for villin indicated that the distribution of the mRNA did not fully parallel that of the protein, although the overall labelling pattern for mRNA and protein along the crypt-villus axis was similar. While villin was present in equal amounts in all cells along the villi, villin-specific mRNA was mainly accumulated in the cells at the villus base, the area of the epithelium where terminal differentiation takes place and where the brush border is formed.

(PDF emailed within 1 workday: $29.90)

Accession: 007208865

Download citation: RISBibTeXText

Related references

Differential distribution of villin and villin MRNA in mouse intestinal epithelial cells. Differentiation; Research in Biological Diversity 39(1): 51-57, 1988

Cloning of mouse villin and examination of villin and gelsolin expression during epithelial differentiation. Journal of Cell Biology 109(4 PART 2): 347A, 1989

Effects of villin protein over-expression in villin-deficient renal epithelial cells. Molecular Biology of the Cell 6(SUPPL ): 24A, 1995

Shigella flexneri infection is dependent on villin in the mouse intestine and in primary cultures of intestinal epithelial cells. Cellular Microbiology 7(8): 1109-1116, 2005

Sea urchin egg villin: identification of villin in a non-epithelial cell from an invertebrate species. Journal of Cell Science 100: 61-71, 1991

Characterization of villin from the intestinal brush border of the rat, and comparative analysis with avian villin. Cell Motility and the Cytoskeleton 9(1): 60-72, 1988

Differential localization of villin and fimbrin during development of the mouse visceral endoderm and intestinal epithelium. Development 106(2): 407-419, 1989

The actin binding protein villin an early differentiation marker of intestinal epithelial cells. Nunez, J , J E Dumont And R Denton (Ed ) Colloque Inserm (Institut National De La Sante Et De La Recherche Medicale), Vol 176 Hormones Et Regulation Cellulaire; (Inserm (National Institute Of Health And Medical Research) Colloquium, Vol 176) Hormones And Cell Regulation; 13th European Symposium, Mont Sainte-Odile, France, September 1988 Xix+151p Les Editions Inserm: Paris, France; John Libbey And Co Ltd : London, England, Uk Illus Paper 53-64, 1989

Suppression of villin expression by antisense RNA impairs brush border assembly in polarized epithelial intestinal cells. Embo Journal 14(3): 409-421, 1995

Suppression of villin expression by antisense Rna impairs brush border assembly in polarized epithelial intestinal cells. The Embo Journal 14(3): 409-421, 1995

Decreased concentrations of saccharase-isomaltase and villin in intestinal epithelial cells of Crohns disease and ulcerative colitis. Gastroenterology 118(4 Suppl 2 Part 2): AGA A1118, 2000

Systemic inflammation and decreased survival in conditional intestinal epithelial cell IKKbeta knockout mice generated using a villin-cre transgenic mouse line. Gastroenterology 122(4 Suppl 1): A 23, 2002

Villin associates with specific micro filamentous structures as seen by immuno fluorescence microscopy on tissue sections and cells micro injected with villin. Experimental Cell Research 135(1): 213-220, 1981

Apical invasion of intestinal epithelial cells by Salmonella typhimurium requires villin to remodel the brush border actin cytoskeleton. Cell Host and Microbe 17(2): 164-177, 2015

The amino-terminal domain of villin Molecular analysis of villin-actin interaction. Biophysical Journal 64(2 PART 2): A124, 1993