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Differential distribution of sodium potassium atpase alpha isoform messenger rnas in the peripheral nervous system



Differential distribution of sodium potassium atpase alpha isoform messenger rnas in the peripheral nervous system



Brain Research 546(1): 47-54



mRNA transcripts for 3 isoforms of the .alpha. subunit of (Na,K)-ATPase have been previously identified in the nervous system (designated .alpha.1, .alpha.2 and .alpha.3). In order to study the localization and expression of the different .alpha. isoforms in the peripheral nervous system, we prepared probes from the unique 3' untranslated region of .alpha.1 cDNA, and from the translated region of .alpha.3 cDNA. These probes were used in dot blot and in situ hybridization assays of rat spinal cord, dorsal root ganglia (DRG), and sciative nerve. Within the ventral horn of lumbar spinal cord, .alpha.1 mRNA was found in a discrete set of laterally placed motor neurons, while .alpha.3 was found in all the identified neurons of the spinal cord, including those motor neurons containing .alpha.1. In the lumbar DRG, .alpha.3 was uniformly distributed in DRG neurons, while .alpha.1 was abundant in some neurons but little or none was found in other neurons. Satellite cells contained neither isoform. Schwann cells in sciatic nerve were labeled with the .alpha.1 probe in a perinuclear distribution, but contained no detectable .alpha.3. Dot blot analysis showed .alpha.1 and .alpha.3 in spinal cord and DRG, but only .alpha.1 in peripheral nerve. These results imply that:(1) the riboprobes are able to distinguish .alpha.1 from .alpha.3 isoform mRNAs under in situ hybridization conditions, (2) both .alpha.1 and .alpha.3 isoforms of (Na,K)-ATPase are found in neurons, (3) some motor and sensory neurons contain abundant .alpha.3 with little or no detectable .alpha.1, while others contain abundant .alpha.1 and .alpha.3, (4) no neurons in the lumbar spinal cord or DRG contain detectable .alpha.1 in the absence of .alpha.3, and (5) Schwann cells contain the .alpha.1 isoform but no detectable .alpha.3 isoform mRNA.

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Accession: 007208904

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