+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Differential involvement of the arachidonic acid cascade on the alpha 1 adrenergic potentiation of vasoactive intestinal peptide versus beta adrenergic stimulated cyclic amp and cyclic gmp accumulation in rat pinealocytes



Differential involvement of the arachidonic acid cascade on the alpha 1 adrenergic potentiation of vasoactive intestinal peptide versus beta adrenergic stimulated cyclic amp and cyclic gmp accumulation in rat pinealocytes



Journal of Neurochemistry 57(5): 1534-1539



In the rat pineal gland, .alpha.1-adrenergic agonists, which stimulate arachidonic acid release, also potentiate vasoactive intestinal peptide (VIP)- or .beta.-adrenergic-stimulated cyclic AMP (cAMP) and cyclic GMP (cGMP) accumulation. In this study, the possible involvement of the arachidonic acid pathway in the potentiation mechanism was examined in dispersed rat pinealocytes using two inhibitors of the arachidonic acid cascade, indomethacin and nordihydroguaiaretic acid. These two inhibitors appeared to have differential effects on the .alpha.1-adrenergic potentiation of VIP- or .beta.-adrenergic-stimulated cAMP and cGMP responses. Whereas, nordihydroguaiaretic acid was effective in suppressing both the .alpha.1-adrenergic potentiation of VIP- or .beta.-adrenergic-stimulated cAMP and cGMP responses, indomethacin inhibited selectivity the VIP-mediated cAMP and cGMP responses. The role of arachidonic acid metabolites was further determined using several prostaglandins - A2, I2, E2, and F2.alpha. - and leukotrienes - B4, C4, and D4. Of the seven compounds tested, prostaglandins E2 and F2.alpha. stimulated basal cAMP but not cGMP accumulation. The prostaglandin E2- and F2.alpha.-stimulated cAMP responses were additive to those stimulated by VIP or .beta.-adrenergic receptors. The other five compounds had no effects on basal or VIP- or .beta.-adrenergic-stimulated cAMP or cGMP accumulation. Taken together, these findings indicate that the arachidonic acid cascade is likely involved in the .alpha.1-adrenergic potentiation of VIP- or .beta.-adrenergic-stimulated cAMP and cGMP accumulation. However, the specific arachidonic acid metabolite involved in the potentiation mechanisms of VIP- versus .beta.-adrenergic-stimulated cyclic nucleotide responses may be different.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 007210424

Download citation: RISBibTeXText

PMID: 1655977


Related references

Pertussis toxin does not inhibit alpha 1-adrenergic potentiation of beta-adrenergic stimulation of cyclic AMP accumulation in rat pinealocytes. Biochemical and Biophysical Research Communications 168(2): 871-877, 1990

Neuropeptide Y inhibits beta-adrenergic agonist- and vasoactive intestinal peptide-induced cyclic AMP accumulation in rat pinealocytes through pertussis toxin-sensitive G protein. Journal of Neurochemistry 59(6): 2178-2183, 1992

Ceramide selectively inhibits calcium-mediated potentiation of beta-adrenergic-stimulated cyclic nucleotide accumulation in rat pinealocytes. Biochemical and Biophysical Research Communications 244(1): 57-61, 1998

Dual receptor regulation of cyclic nucleotides: alpha 1-adrenergic potentiation of vasoactive intestinal peptide stimulation of pinealocyte adenosine 3',5'-monophosphate. Endocrinology 122(4): 1646-1651, 1988

See-saw signal processing in pinealocytes involves reciprocal changes in the alpha 1-adrenergic component of the cyclic GMP response and the beta-adrenergic component of the cyclic AMP response. Journal of Neurochemistry 47(3): 678-686, 1986

Augmentation and inhibition of beta-adrenergic agonists-stimulated tissue cyclic AMP accumulation by alpha-adrenergic agonists in rat parotid gland. Japanese Journal of Physiology 37(5): 881-897, 1987

Alpha-2 adrenergic inhibition of intestinal secretion induced by prostaglandin E1, vasoactive intestinal peptide and dibutyryl cyclic AMP in rat jejunum. Journal of Pharmacology and Experimental Therapeutics 220(3): 637-641, 1982

Interaction of melatonin with human lymphocytes: evidence for binding sites coupled to potentiation of cyclic AMP stimulated by vasoactive intestinal peptide and activation of cyclic GMP. Journal of Pineal Research 12(3): 97-104, 1992

Alfa 1 adrenergic potentiation of progesterone accumulation stimulated by vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating polypeptide (PACAP) in cultured rat granulosa cells. Neuro Endocrinology Letters 23(2): 141-148, 2002

Inhibitory effects of ethanol on the calcium-dependent potentiation of vasoactive intestinal peptide-stimulated cAMP and cGMP accumulation in rat pinealocytes. Biochemical Pharmacology 42(8): 1601-1608, 1991

Cyclic AMP response to vasoactive intestinal peptide and beta-adrenergic or cholinergic agonists in isolated epithelial cells of rat ventral prostate. Bioscience Reports 5(9): 791-797, 1985

Differential effects of intracellular calcium elevating agents on adrenergic-stimulated cyclic nucleotide and melatonin synthesis in rat pinealocytes. Canadian Journal Of Physiology & Pharmacology. 70(9): 1254-1260, 1992

Effects of alpha adrenergic and beta adrenergic agonists and antagonists on extracellular recorded action potential and on the accumulation of cyclic amp in rat superior cervical ganglion in vitro. George, William J And Louis J Ignarro (Ed ) Advances in Cyclic Nucleotide Research, Vol 9 Third International Conference on Cyclic Nucleotides New Orleans, La , Usa, July 17-22, 1977 Xxxii+799p Illus Raven Press: New York, N Y , Usa ISBN 0-89004-240-3: 745-746, 1978

Down regulation of pinealocyte protein kinase c effect on alpha 1 adrenergic potentiation of beta adrenoceptor stimulation of cyclic amp accumulation and induction of serotonin n acetyltransferase activity. Journal of Neurochemistry 57(1): 216-221, 1991

Alpha adrenergic involvement in heart metabolism effects on cyclic amp cyclic amp dependent protein kinase cyclic gmp and glucose transport. Molecular Pharmacology 13(5): 965-975, 1977