Differentiation in callus cultures of a tobacco nicotiana tabacum cultivar white burley variant some biochemical aspects

Kumar, V.; Maherchandani, N.

Plant Cell Tissue and Organ Culture 14(3): 177-186


ISSN/ISBN: 0167-6857
Accession: 007211964

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A slow-growing variant plant with distinct foliar and floral morphology was obtained in tissue cultures of Nicotiana tabacum cv. White Burley. The root and shoot differentiation in the callus derived from normal plants occurred on the 8th and 12th day, respectively, but took 10 and 14 days, respectively, in the variant callus. Amylase and acid phosphatase activities, starch and soluble carbohydrate contents were studied in non-differentiating callus (NDC), root differentiating callus (RDC) and shoot differentiating callus (SDC). The activities of amylase and acid phosphatase were low in the variant as compared to normal. Maximum amylase and acid phosphatase activities coincided with the appearance of roots or shoots. There was more starch accumulation in normal callus on differentiating media, but the variant showed a less pronounced change. The normal callus under differentiating conditions also showed more increase in soluble carbohydrates as compared to the variant. The increase in soluble carbohydrate was maintained till roots and shoot appeared. The lag exhibited by the variant in differentiating was reflected in slow development of enzyme activities and low starch and sugar concentrations.