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Directionality of dnaa protein dna interaction active orientation of the dnaa protein dna box complex in transcription termination



Directionality of dnaa protein dna interaction active orientation of the dnaa protein dna box complex in transcription termination



EMBO (European Molecular Biology Organization) Journal 8(5): 1609-1614



The complex of DnaA protein with its 9 bp consensus binding site, the dnaA box 5'-TT(A/T)T(A/C)CA(A/C)A, blocks transcribing RNA polymerase. In a model system, the rate of transcription was monitored distal to the dnaA box 5'-TTTTCCACA by the expression of a reporter gene. DnaA-dependent transcription termination occurred irrespective of whether the dnaA box region was or was not translated. Only the dnaA box orientation 5'-TTTTCCACA on the non-coding strand, but not the reverse orientation, was active in termination. This suggest that DnaA protein contacts only one strand of the DNA duplex. Oligonucleotide-directed mutation of a dnaA box present within the dnaA coding region resulted in increased expresion of dnaA. This demonstrates that DnaA protein-directed transcription termination is an element of the autoregulation of the dnaA gene.

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Accession: 007215880

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PMID: 2548849


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