+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Effect of genotype and embryo age on wheat immature embryo response in in vitro culture

Effect of genotype and embryo age on wheat immature embryo response in in vitro culture

Acta Genetica Sinica 16(2): 81-88

Thirty-four genotypes of Triticum aestivum were comparatively examined with regard to thier immature embryo response in in vitro culture. Callus induction frequency of all the genotypes tested varied from 100% to 71% with an average of 92.4%. Since such a moderate variation may be attributed to either environmental factors conditioning the physiological state of the donor plants, or artificial factors imposed during the experimental manipulation, it seems that no substantial differences exist among the genotypes in terms of their response to callus induction. In contrast, regenerable or shoot-forming callus frequency varied remarkably with genotype, ranging from 60% to 0% with an average of 32.7%. Although, for a given genotype, it also varied from year to year, the presence or absence of the regenerability seems to be a stable genetic attribute. Therefore, response to callus induction and regenerability of the callus in culture of immature embryo of wheat may be conditioned by different genetic mechanisms. In Spite of the optimal embryo size for in vitro culture seeming to be about 1 mm in length, upon a longer period of time in culture, scutellar callus could even be successfully initiated with embryos as small as 0.3 mm. Callus induction frequency and differentiation frequency in the latter case appeared as high as about 100% and 60%, respectively.

(PDF emailed within 1 workday: $29.90)

Accession: 007248462

Download citation: RISBibTeXText

Related references

Effect of genotype on the response in immature embryo culture of japanese and chinese wheat. Science Reports of Faculty of Agriculture Kobe University 18(2): 165-172, 1989

Effects of genotype and embryo age on the response of in vitro cultured immature embryos of wheat. Acta Genetica Sinica 16(2): 81-88, 1989

Embryo culture technique. I. The growth of immature tomato embryo in vitro. II. Embryo factors and immature tomato embryo. III. Growth of hybrid embryos (Lycopersicon esculentum x Lycopsrsicon peruvianum) in cuitare medium. Indian J. Hort, 12: 143-58, 1955

Embryo culture technique - I The growth of immature tomato embryo in vitro II Embryo factors and immature tomato embryo III Growth of hybrid embryos in culture medium. 1955

Response of durum wheat cultivars to immature embryo culture, callus induction and in vitro salt stress. Plant cell tissue and organ culture8(1): 67-72, 1999

Studies on immature embryo culture of peanut (Arachis hypogaea L.). III. In vitro organogenesis from immature embryo axes and cotyledons. Journal of Agriculture and Forestry 46(3): 39-51, 1997

A unique Aegilops tauschii genotype needless to immature embryo culture in cross with wheat. Acta Botanica Sinica 44(6): 708-713, June, 2002

Effect of genotype and culture medium on morphogenesis of linseed (Linum usitatissimum L.) immature embryo culture. Plant Cell Biotechnology and Molecular Biology 5(3/4): 155-160, 2004

In vitro culture of soybean immature embryo VII. Histological studies organogenesis and embryogenesis from soybean immature embryo cultures. Journal of the Agricultural Association of China 0(166): 6-15, 1994

Genetic effects in wheat immature embryo culture response. Hereditas Beijing 13(6): 34-37, 1991

Study on in vitro technique for immature embryo culture of wheat breeding. Acta Agronomica Sinica 14(2): 137-142, 1988

Studies on response of wheat immature embryo culture to gamma ray irradiation. Genetic Manipulation in Crops Newsletter 2(1): 19-26, 1986

Effects of casein hydrolysate on the response in immature embryo culture wheat triticum aestivum l. Japanese Journal of Breeding 42(2): 367-373, 1992