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Effect of genotype and embryo age on wheat immature embryo response in in vitro culture



Effect of genotype and embryo age on wheat immature embryo response in in vitro culture



Acta Genetica Sinica 16(2): 81-88



Thirty-four genotypes of Triticum aestivum were comparatively examined with regard to thier immature embryo response in in vitro culture. Callus induction frequency of all the genotypes tested varied from 100% to 71% with an average of 92.4%. Since such a moderate variation may be attributed to either environmental factors conditioning the physiological state of the donor plants, or artificial factors imposed during the experimental manipulation, it seems that no substantial differences exist among the genotypes in terms of their response to callus induction. In contrast, regenerable or shoot-forming callus frequency varied remarkably with genotype, ranging from 60% to 0% with an average of 32.7%. Although, for a given genotype, it also varied from year to year, the presence or absence of the regenerability seems to be a stable genetic attribute. Therefore, response to callus induction and regenerability of the callus in culture of immature embryo of wheat may be conditioned by different genetic mechanisms. In Spite of the optimal embryo size for in vitro culture seeming to be about 1 mm in length, upon a longer period of time in culture, scutellar callus could even be successfully initiated with embryos as small as 0.3 mm. Callus induction frequency and differentiation frequency in the latter case appeared as high as about 100% and 60%, respectively.

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Accession: 007248462

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