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Effects of gamma interferon tumor necrosis factor alpha and interleukin 2 on infection and proliferation of theileria parva infected bovine lymphoblasts and production of interferon by parasitized cells

Effects of gamma interferon tumor necrosis factor alpha and interleukin 2 on infection and proliferation of theileria parva infected bovine lymphoblasts and production of interferon by parasitized cells

Infection and Immunity 59(12): 4540-4546

Theleria parva is a protozoan parasite that infects bovine B cells and .alpha.beta. and .gamma.delta. T cells and transforms them into continually proliferating cells. CD4+ T. parva-antigen-specific immune T cells have been shown to produce cytokines in response to stimulation with parasitized cells, and T. parva-infected lymphocytes produce and consume T-cell growth factors and interleukin-2 (IL-2). To ascertain the role of T-cell cytokines on T. parva infections, we evaluated recombinant gamma interferon (rIFN-.gamma.), rIL-2, and tumor necrosis factor alpha (rTNF-.alpha.) for their effects on establishment, proliferation, and survival of parasitized cells. The results indicate that neither rIF-.gamma. nor rTNF-.alpha. had an enhancing or inhibitory effect on the growth of established T. parva-infected T-cell clones, whereas bovine rIL-2 increased the proliferation of infected B-cell and .alpha.beta. T-cell clones but not that of .gamma.delta. T-cell clones. To evaluate the effects of the cytokines on establishment of parasitized cell lines, peripheral blood mononuclear cells were cultured in their presence immediately following infection with T. parva sporozites. Neither rIFN-.gamma. nor rIL-2 altered the proportion of cells initially developing schizonts, but both enhanced establishment of infected cell lines by about twofold. In contrast, rTNF-.alpha. resulted in about a 33% decrease in the proportion of schizont-infected cells. Inhibitory effects on establishment of parasitized cell lines by rTNF-.alpha. were no longer apparent by 12 days following infection. Tests conducted during this study indicated that T. parva-infected lymphocytes also spontaneously produce IFN that is neutralized by acidic pH treatment. In conclusion, we speculate that none of these T-cell cytokines are likely to have a profound inhibitory effect in vivo on T. parva infections. Instead, IFN-.gamma. and IL-2 may facilitate the establishment of infection by T. parva.

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Accession: 007275213

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PMID: 1937812

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