+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Effects of gamma interferon tumor necrosis factor alpha and interleukin 2 on infection and proliferation of theileria parva infected bovine lymphoblasts and production of interferon by parasitized cells



Effects of gamma interferon tumor necrosis factor alpha and interleukin 2 on infection and proliferation of theileria parva infected bovine lymphoblasts and production of interferon by parasitized cells



Infection and Immunity 59(12): 4540-4546



Theleria parva is a protozoan parasite that infects bovine B cells and .alpha.beta. and .gamma.delta. T cells and transforms them into continually proliferating cells. CD4+ T. parva-antigen-specific immune T cells have been shown to produce cytokines in response to stimulation with parasitized cells, and T. parva-infected lymphocytes produce and consume T-cell growth factors and interleukin-2 (IL-2). To ascertain the role of T-cell cytokines on T. parva infections, we evaluated recombinant gamma interferon (rIFN-.gamma.), rIL-2, and tumor necrosis factor alpha (rTNF-.alpha.) for their effects on establishment, proliferation, and survival of parasitized cells. The results indicate that neither rIF-.gamma. nor rTNF-.alpha. had an enhancing or inhibitory effect on the growth of established T. parva-infected T-cell clones, whereas bovine rIL-2 increased the proliferation of infected B-cell and .alpha.beta. T-cell clones but not that of .gamma.delta. T-cell clones. To evaluate the effects of the cytokines on establishment of parasitized cell lines, peripheral blood mononuclear cells were cultured in their presence immediately following infection with T. parva sporozites. Neither rIFN-.gamma. nor rIL-2 altered the proportion of cells initially developing schizonts, but both enhanced establishment of infected cell lines by about twofold. In contrast, rTNF-.alpha. resulted in about a 33% decrease in the proportion of schizont-infected cells. Inhibitory effects on establishment of parasitized cell lines by rTNF-.alpha. were no longer apparent by 12 days following infection. Tests conducted during this study indicated that T. parva-infected lymphocytes also spontaneously produce IFN that is neutralized by acidic pH treatment. In conclusion, we speculate that none of these T-cell cytokines are likely to have a profound inhibitory effect in vivo on T. parva infections. Instead, IFN-.gamma. and IL-2 may facilitate the establishment of infection by T. parva.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 007275213

Download citation: RISBibTeXText

PMID: 1937812


Related references

Interleukin-2 production and expression of interleukin-2 receptor by activated peripheral blood T-lymphocytes in Hodgkin's disease. Journal of the Medical Research Institute 17(1): 157-166, 1996

Production of gamma delta t cells by the adult murine thymus. Journal of Cellular Biochemistry Suppl. (16 Part D): 70, 1992

In vitro induction/inhibition of germinal vesicle breakdown (GVBD) in frog (Euphlyctis cyanophlyctis) oocytes by endocrine active compounds. Drug and Chemical Toxicology 36(2): 217-223, 2013

Synthetic peptides corresponding to protective epitopes of Escherichia coli digalactoside-binding pilin prevent infection in a murine pyelonephritis model. Proceedings of the National Academy of Sciences of the United States of America 85(4): 1247-1251, 1988

Production of tumor necrosis factor-alpha by naive or memory T lymphocytes activated via CD28. Cellular Immunology 139(1): 198-207, 1992

Acute hematologic and hemorheologic effects of sulfur dioxide inhalation. Archives of Environmental Health 43(5): 344-348, 1988

Effects of cytokines ifn alpha ifn gamma tnf and il 1 on monocyte functions. Friedman, R M , T Merigan And T Sreevalsan (Ed ) Ucla (University Of California-Los Angeles) Symposia on Molecular And Cellular Biology New Series, Vol 50 Interferons As Cell Growth Inhibitors And Antitumor Factors; Schering Corporation-Ucla Symposium, Steamboat Springs, Colorado, Usa, April 6-12, 1986 Xxxviii+541p Alan R Liss, Inc : New York, New York, Usa Illus 197-208, 1986, 1987

Interleukin 1 and tumor necrosis factor alpha do not regulate protein balance in skeletal muscle. Journal of Leukocyte Biology 42(5): 549, 1987

Interleukin-1-alpha and tumor necrosis factor-alpha induce mannose receptor expression in glomerular mesangial cells. Journal of the American Society of Nephrology 5(3): 756, 1994

Development of four tobacco cytoplasmic male sterile sources using in vitro techniques. Plant Cell Tissue and Organ Culture. 27(3): 289-295, 1991

Overlapping of the transcription initiation and dna binding sites. Investigative Ophthalmology and Visual Science 29(ABSTR ISSUE): 424, 1988

Amelioration of cisplatin toxicity with two different antidotes. Journal of the Medical Research Institute 20(4): 18-32, 1999

Heterogeneous response of hepatic mixed function oxidases to chronic phenobarbital administration. Biochemical Pharmacology 28(6): 849-857, 1979

Early interactions, adhesion, and establishment of the infection court by Erysiphe graminis. Canadian Journal Of Botany. 73(Suppl. 1): S609-S615, 1995

Triggering of the great toe. A case report. Clinical Orthopaedics and Related Research 1983(175): 184-185, 1983