Effects of human platelet transforming growth factor beta on replication and collagen synthesis in primarily cultured mesangial cell

Cho S W.; Park S K.; Kim G Y.; Lee, H.; Lee, J.D.

Korean Biochemical Journal 25(3): 189-195

1992


ISSN/ISBN: 0368-4881
Accession: 007276392

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Abstract
The effect of TGF-.beta. on cell replication and collagen synthesis in primarily cultured mesangial cells from rats was studied in vitro. The TGF-.beta. was purified from human platelets by acidic ethanol extraction and gel filtration with FPLC. The effect of TGF-.beta. on the DNA synthesis and the collagen synthesis in mesangial cells depended on both TGF-.beta. concentration and cell density in monolayer culture. Concentrations of TGF-.beta. ranging from 0.1 .apprx. 15 ng/ml were evaluated for their effects upon DNA synthesis. In confluent cells, when compared to the growth rate in standard medium, TGF-.beta. concentrations in the range 0.1 .apprx. 15 ng/ml reduced a cell proliferation up to 35%, suggesting inhibition of cell division. The effect of TGF-.beta. on collagen synthesis in mesangial cells was biphasic. In confluent cells, the addition of TGF-.beta. induced a 2-fold increase in the production of collagen when compared with cells grown in standard medium. The maximal effect of TGF-.beta. occured at 0.5 ng/ml. At higher concentrations there was a decrease in the action of TGF-.beta. with the effects on collagen production. In subconfluent cells, however, TGF-.beta. showed no significant effects on the DNA synthesis and the collagen synthesis at all concentrations. These results support that TGF-.beta. may play an important role as multifunctional regulator in the pathogenesis of glomerulosclerosis.