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Effects of trialkyltin chlorides on isolated rat hepatocytes

Yamada, J.

Agricultural and Biological Chemistry 55(9): 2313-2320

1991


ISSN/ISBN: 0002-1369
DOI: 10.1271/bbb1961.55.2313
Accession: 007287761

Effects of trialkyltin chlorides on isolated rat hepatocytes were examined. Incubation of isolated hepatocytes with trialkyltin chloride at concentrations of 1-100 .mu.g/ml for a 2-hr period resulted in a rapid decrease of cell viability. While both triethyl- and tributyltin chloride intensely inhibited oxygen uptake in isolated hepatocytes, tributyltin chloride proved to be much more toxic than triethyltin chloride. Triethyltin chloride strongly inhibited mitochondrial respiration at the low concentrations. Cells treated with tributyltin chloride leaked large amounts of magnesium, protein, and 260-nm-absorbing materials. Cells treated with triethyltin chloride also showed some leakages, but to a lesser degree. Electron microscopic observation showed that the surfaces and intracellular structures of isolated hepatocytes were badly damaged by tributyltin chloride.

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