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Elimination of apple mosaic virus and raspberry bushy dwarf virus from infected red raspberry rubus idaeus l. by tissue culture

Elimination of apple mosaic virus and raspberry bushy dwarf virus from infected red raspberry rubus idaeus l. by tissue culture

Journal of Phytopathology (Berlin) 127(3): 193-199

Experiments with ApMV infected 'Malling Landmark' and RBDV infected 'Schonemann' and 'Trent' plants were carried out to evaluate a) the dependence of virus eradication on explant size and mass propagation, b) the reliability of results of ELISA tests on in vitro plantlets. With ApMV a correlation between virus elimination and explant size was observed, whereas with RBDV even plantlets from the smallest established explants were still infected. With ApMV, in vitro multiplication for three subsequent subcultures did not lead to further virus elimination, with RBDV this was observed in two cases. ELISA test results for both viruses, ApMV and RBDV, were identical when small in vitro plantlets, long-term stored plants, or potted plants from the same origin were tested, indicating that virus tests are possible with very young plant material and can be used to select virus-free plants in vitro. Tissue culture permits long-term storage of plant viruses. It is also suitable for plant virus propagation and could be a useful aid in plant virus purification. For commercial multiplication only virus-indexed plant material should be used for establishment and further propagation in vitro.

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Accession: 007296491

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DOI: 10.1111/j.1439-0434.1989.tb01129.x

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