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Evaluation of genotoxic potential of ethylene dibromide using genetic systems of higher plants



Evaluation of genotoxic potential of ethylene dibromide using genetic systems of higher plants



Asian Journal of Plant Science 1(1): 77-88



Genotoxic potential of ethyelne dibromide (EDB) has been studied using cytogenetic, genetic and in vitro end points of higher plants. EDB induced a wide variety of cytogenetic effects in Allium root assay. The specific locus mutation assay, involving the waxy locus (Wx-wx), did not reveal any mutation event, although a high frequency of pollen sterility was noted. On the other hand, the multilocus tests comprising chlorophyll mutations and the variability for metric traits have indicated mutagenic potential of EDB, when compared to the positive mutagen, ethylmethane sulphonate (EMS). The in vitro assay of callus growth suggested that EBD effects the cell growth and metabolism. The study also indicated a positive concordance between different genetic end points, suggesting that, any one or combinations of bioassays could be employed for monitoring the genotoxic effects of environmental pollutants.

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